Back to Search
Start Over
Efficient ammonia production from food by-products by engineered Escherichia coli
- Source :
- AMB Express, AMB Express, Vol 10, Iss 1, Pp 1-9 (2020)
- Publication Year :
- 2020
- Publisher :
- Springer Berlin Heidelberg, 2020.
-
Abstract
- Ammonia is used as a fertilizer for agriculture, chemical raw material, and carrier for transporting hydrogen, and with economic development, the demand for ammonia has increased. The Haber–Bosch process, which is the main method for producing ammonia, can produce ammonia with high efficiency. However, since it consumes a large amount of fossil energy, it is necessary to develop an alternative method for producing ammonia with less environmental impact. Ammonia production from food by-products is an appealing production process owing to unused resource usage, including waste, and mild reaction conditions. However, when food by-products and biomass are used as feedstocks, impurities often reduce productivity. Using metabolic profiling, glucose was identified as a potential inhibitor of ammonia production from impure food by-products. We constructed the recombinant Escherichia coli, in which glucose uptake was reduced by ptsG gene disruption and amino acid catabolism was promoted by glnA gene disruption. Ammonia production efficiency from okara, a food by-product, was improved in this strain; 35.4 mM ammonia was produced (47% yield). This study might provide a strategy for efficient ammonia production from food by-products.
- Subjects :
- 0106 biological sciences
lcsh:Biotechnology
lcsh:QR1-502
Biophysics
engineering.material
Raw material
medicine.disease_cause
01 natural sciences
Applied Microbiology and Biotechnology
lcsh:Microbiology
Metabolic engineering
Ammonia production
03 medical and health sciences
Ammonia
chemistry.chemical_compound
lcsh:TP248.13-248.65
010608 biotechnology
medicine
Metabolic profiling
Escherichia coli
Food by-products
030304 developmental biology
0303 health sciences
Chemistry
Catabolism
Biorefinery
Pulp and paper industry
engineering
Original Article
Fertilizer
Subjects
Details
- Language :
- English
- ISSN :
- 21910855
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- AMB Express
- Accession number :
- edsair.doi.dedup.....e5d42343dbfbf1c8eb0518d4e888ffd8