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Deep UV sensing of the interaction of porphyrin with bovine serum albumin protein
- Publication Year :
- 2009
- Publisher :
- Elsevier, 2009.
-
Abstract
- The interaction of meso-tetrakis(p-sulfonatophenyl)porphyrin (TPPS) with bovine serum albumin (BSA) in neutral solution (pH 7.4) has been studied by means of absorption, steady-state fluorescence and time-resolved fluorescence spectroscopy. The formation of TPPS–BSA complex was monitored by spectroscopic characteristic changes in Soret band absorption and fluorescence emission of TPPS. Applying the time-correlated single-photon counting (TCSPC) method, BSA–TPPS interaction was also investigated by the fluorescence lifetime of tryptophan residues in BSA. The results demonstrate that deep UV laser-based fluorescence lifetime microscopy is useful for sensitive identification of protein interaction using intrinsic fluorescence.
- Subjects :
- 10120 Department of Chemistry
3104 Condensed Matter Physics
2506 Metals and Alloys
Fluorescence in the life sciences
Photochemistry
Fluorescence spectroscopy
540 Chemistry
Materials Chemistry
Electrical and Electronic Engineering
Bovine serum albumin
Laser-induced fluorescence
Instrumentation
2505 Materials Chemistry
biology
Chemistry
3105 Instrumentation
2208 Electrical and Electronic Engineering
Metals and Alloys
2508 Surfaces, Coatings and Films
2504 Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Fluorescence
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Resonance fluorescence
biology.protein
Fluorescence cross-correlation spectroscopy
Time-resolved spectroscopy
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....e5ddfe353ac286daa4f7957d29c61886
- Full Text :
- https://doi.org/10.5167/uzh-28443