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Genotoxic potential of TiO(2) on bottlenose dolphin leukocytes
Genotoxic potential of TiO(2) on bottlenose dolphin leukocytes
- Publication Year :
- 2010
-
Abstract
- Titanium dioxide is extensively used in a variety of products, including industrial materials and cosmetics. Studies mainly performed on human cell lines and in vivo exposure on experimental animals have raised concern about the toxic effects of ultrafine titanium dioxide; however, scarce information is available about its impact on aquatic life. The aim of this article was to assess the genotoxic potential of TiO(2) (anatase and rutile) on bottlenose dolphin leukocytes. Blood samples were obtained from four male and one female specimens reared at the Adriatic SeaWorld "Oltremare" (Riccione, Italy). Leukocytes were isolated by the lyses procedure and in vitro exposed to TiO(2) in RPMI. Experimental solutions were sonicated immediately before dosing the cells. Three exposure times (4, 24 and 48 h) and three doses (20, 50 and 100 microg/ml) were tested. Genotoxicity was detected by the single-cell gel electrophoresis (or comet assay) at pHor = 13, assessing single/double-strand breaks and alkali-labile sites. Cytotoxicity was also detected by the Trypan blue exclusion method. Results showed that both the crystalline forms of TiO(2) were genotoxic for bottlenose dolphin leukocytes, with a statistically significant increase of DNA fragmentation after exposure to 50 and 100 microg/ml for 24 and 48 h. Although preliminary, these are the first data regarding the genetic susceptibility of toothed cetaceans toward an "emerging" pollutant, such as TiO(2) particles.
- Subjects :
- Male
Cell Survival
DNA Fragmentation
medicine.disease_cause
Biochemistry
Analytical Chemistry
Toxicology
chemistry.chemical_compound
In vivo
Leukocytes
medicine
Animals
Cytotoxicity
Cells, Cultured
Titanium
biology
Mutagenicity Tests
Chemistry
Bottlenose dolphin
biology.organism_classification
Molecular biology
Bottle-Nosed Dolphin
Comet assay
Toxicity
DNA fragmentation
Female
Trypan blue
Genotoxicity
Mutagens
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....e5ea3f81b572903c59a5b952535a388b