Identification of soybean peptides and their effect on the growth and metabolism of Limosilactobacillus reuteri LR08

Lactic acid bacteria are one of the most pivotal probiotics. Promoting their viability could be an effective method to modulate the balance of intestinal flora, thereby improving human health. The current solution is to take advantage of carbon-sourced prebiotics, while protein and peptides with potential prebiotic functions have not been investigated. Soy proteins and peptides have been proven to enhance the growth and metabolism of Lactobacillus. However, research on the relationship between strains of lactobacilli and the structure of soybean peptides is still limited. In the present study, soybean protein and peptides effectively increased the growth and organic acid secretion of Limosilactobacillus reuteri LR08. Additionally, soybean peptides and fructooligosaccharides showed synergistic effects in modulating Limosilactobacillus reuteri LR08. Fraction 1 acquired from soy peptides using RP-HPLC exhibited the most effectiveness and several novel peptides were identified. These results could theoretically and practically benefit soybean peptide application as a potential prebiotic.