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Class 1 Integrons in Urinary Isolates of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Southern China During the Past Five Years
- Source :
- Microbial Drug Resistance. 19:289-294
- Publication Year :
- 2013
- Publisher :
- Mary Ann Liebert Inc, 2013.
-
Abstract
- We analyzed extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (226) and Klebsiella pneumoniae (53) collected from urine specimens during 2005-2009 for the presence of ESBL genes, class 1 integrons, and characterization of gene cassettes. TEM and CTX-M β-lactamase genes were the most prevalent. One hundred and forty-four E. coli and 35 K. pneumoniae were positive for the class 1 integrase gene; among them, 99 E. coli and 14 K. pneumoniae detected gene cassettes. Gene cassette regions were identified by restriction fragment length polymorphism and DNA sequencing analysis. Eleven distinct gene cassette arrays were found in E. coli isolates, and seven distinct gene cassette arrays were found in K. pneumoniae isolates. The cassette array aacA4-catB8-aadA1 was first found in E. coli and dfrA1-orfC was first reported in K. pneumoniae. Most of the gene cassettes found in this class 1 integrons were for aminoglycoside resistance. Enterobacterial repetitive intergenic consensus-polymerase chain reaction fingerprint patterns revealed the isolates carrying gene cassettes were genetically unrelated. In conclusion, we studied the class 1 integrons among urinary isolates of ESBL-producing E. coli and K. pneumoniae in Southern China during the past 5 years and found that class 1 integrons were widely disseminated and played a major role in antibiotic resistance.
- Subjects :
- DNA, Bacterial
Microbiology (medical)
China
Klebsiella pneumoniae
Immunology
Microbial Sensitivity Tests
beta-Lactams
medicine.disease_cause
Polymerase Chain Reaction
Microbiology
beta-Lactamases
DNA sequencing
Integrons
Intergenic region
Drug Resistance, Multiple, Bacterial
Escherichia coli
medicine
Humans
Gene
Escherichia coli Infections
Phylogeny
Pharmacology
Integrases
biology
Sequence Analysis, DNA
biology.organism_classification
Virology
Anti-Bacterial Agents
Klebsiella Infections
Integrase
Aminoglycosides
Gene cassette
Multigene Family
biology.protein
Restriction fragment length polymorphism
Subjects
Details
- ISSN :
- 19318448 and 10766294
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Microbial Drug Resistance
- Accession number :
- edsair.doi.dedup.....e649296ff01d83a9421f0179b9b95539