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In vitro targeting and selective killing of mcf-7 and colo320dm cells by 5-fluorouracil anchored to carboxylated SWCNTs and MWCNTs
- Source :
- Journal of Materials Science. Materials in Medicine
- Publication Year :
- 2021
- Publisher :
- Springer US, 2021.
-
Abstract
- The intention of the present work was to synthesize the f-MWCNT and f-SWCNT terminated with proper functional group, loading of 5-Flurouracil and to perform cytotoxic activity. Functionalization of MWCNTs and SWCNTs was achieved through the acid treatment (H2SO4 + HNO3). 5-flurouracil was loaded into the prepared functionalized CNTs, thereafter; in vitro drug loading capacity and % drug release were calculated. Also the prepared f-CNTs, 5-flurouracil loaded CNTs were distinguished by using SEM, TGA, DSC, X-ray diffraction, Raman and FTIR spectroscopy. MCF-7 and COLO320DM cells were treated with selected concentrations of 5-FU loaded f-MWCNTs and f-SWCNTs to estimate the cytotoxic activity. It was observed that 5-FU loaded f-SWCNTs showed good activity against selected cell lines than others. Moreover, apoptosis percentage was reported to be 84.46 ± 4.3515 and 92.78 ± 2.6549 for 5-FU loaded f-SWCNTs against MCF-7 and COLO320DM cells respectively. It is evident from the results that the prepared drug loaded CNTs have comparable antitumor activity in cancer cell lines.
- Subjects :
- Materials science
Biomedical Engineering
Biophysics
Bioengineering
Apoptosis
02 engineering and technology
In Vitro Techniques
010402 general chemistry
Spectrum Analysis, Raman
01 natural sciences
Nitric Acid
Biomaterials
symbols.namesake
Drug Delivery Systems
X-Ray Diffraction
Cell Line, Tumor
Spectroscopy, Fourier Transform Infrared
Cytotoxic T cell
Humans
Fourier transform infrared spectroscopy
Calorimetry, Differential Scanning
Nanotubes, Carbon
Sulfuric Acids
021001 nanoscience & nanotechnology
In vitro
Chromatin
0104 chemical sciences
Delivery Systems
Drug Liberation
MCF-7
Cell culture
Thermogravimetry
symbols
MCF-7 Cells
Microscopy, Electron, Scanning
Surface modification
Fluorouracil
0210 nano-technology
Raman spectroscopy
Nuclear chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 15734838 and 09574530
- Volume :
- 32
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of Materials Science. Materials in Medicine
- Accession number :
- edsair.doi.dedup.....e69f6cf45cfc6a7823183da647cc9aaa