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TSH/cAMP up-regulate sarco/endoplasmic reticulum Ca2+-ATPases expression and activity in PC Cl3 thyroid cells

Authors :
Sonia Treglia
Francesco Pacifico
Agnese Secondo
Fortunato Moscato
Bruno Di Jeso
Silvestro Formisano
Stefania De Micheli
Luca Ulianich
Domenico Liguoro
Lucio Annunziato
Eduardo Consiglio
Santo Marsigliante
Ulianich, L
Secondo, A
DE MICHELI, S
Treglia, S
Pacifico, F
Liguoro, D
Moscato, F
Marsigliante, Santo
Annunziato, L
Formisano, S
Consiglio, E
DI JESO, Bruno
Ulianich, Luca
Secondo, Agnese
De Micheli, S
Pacifico, FRANCESCO MARIA
Marsigliante, S
Annunziato, Lucio
Formisano, Silvestro
Consiglio, Eduardo
Di Jeso, B.
Source :
European journal of endocrinology 150 (2004): 851–861. doi:10.1530/eje.0.1500851, info:cnr-pdr/source/autori:Ulianich L; Secondo A; De Micheli S; Treglia S; Pacifico F; Liguoro D; Moscato F; Marsigliante S; Annunziato L; Formisano S; Consiglio E; Di Jeso B./titolo:TSH%2FcAMP up-regulate sarco%2Fendoplasmic reticulum Ca2+-ATPases expression and activity in PC Cl3 thyroid cells./doi:10.1530%2Feje.0.1500851/rivista:European journal of endocrinology/anno:2004/pagina_da:851/pagina_a:861/intervallo_pagine:851–861/volume:150
Publication Year :
2004
Publisher :
Oxford University Press (OUP), 2004.

Abstract

OBJECTIVE: We recently reported that the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) 2b is the SERCA form preferentially expressed in rat thyroid. Moreover, SERCA2b expression dramatically decreases in virally transformed, highly tumorigenic, PC Cl3 thyroid cells. These results suggest that, in the thyroid, SERCA2b, in addition to its housekeeping role, is linked to differentiation and is a regulated gene. We therefore sought to study the effect of TSH, the main regulator of thyroid function, on SERCA2b expression and activity. METHODS: PC Cl3 cells were hormone starved in low-serum medium and stimulated for long (48 h) or short (1, 2 and 4 h) times. SERCA2b expression and activity were evaluated by Northern and Western blots, Ca2+-ATPase activity and Ca2+ store content. RESULTS: In PC Cl3 cells, SERCA2b mRNA and protein were induced twofold by a 48-h long treatment with TSH. Long-term elevation (48 h) of intracellular cAMP levels, by forskolin or 8-Br-cAMP, had similar effects on SERCA2b mRNA and protein. We also measured Ca2+-ATPase activity and Ca2+ store content. Both long (48 h) and short (0.5-1 h) treatments with TSH, forskolin or 8-Br-cAMP induced a marked increase of SERCA2b activity. This effect was completely abolished by H89, a specific inhibitor of cAMP-dependent protein kinase A (PKA). TSH and 8-Br-cAMP increased Ca2+ store content after both long (48 h) and short (1-2 h) treatments. CONCLUSIONS: These data suggested that TSH/cAMP acts as an important regulator of both SERCA2b expression and activity in the thyroid system, through PKA activation.

Details

ISSN :
1479683X and 08044643
Database :
OpenAIRE
Journal :
European Journal of Endocrinology
Accession number :
edsair.doi.dedup.....e6bb16543f98dc236577b4610b7bee0a
Full Text :
https://doi.org/10.1530/eje.0.1500851