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Isolation of giant unilamellar vesicles from electroformed vesicle suspensions and their extrusion through nano-pores
- Source :
- IndraStra Global.
- Publication Year :
- 2013
- Publisher :
- ELSEVIER IRELAND LTD, 2013.
-
Abstract
- Liposomes required for drug delivery are commonly obtained by extrusion of phospholipid vesicle suspensions through track-etched membranes. The effects of trans-membrane pressure, membrane pore size and bilayer composition on extruded liposome size are well-studied. Vesicle suspensions used in these extrusion studies are highly polydisperse, ranging from 20 nm to 100 m. Vesicle sub-populations smaller than membrane pore size do not undergo extrusion-mediated size reduction and contribute significantly to the mean radius of extruded liposomes. In the present work, giant unilamellar vesicles (Guys) are isolated by subjecting electroformed vesicle suspensions to low-pressure filtration. The isolated GUVs are extruded through track-etched polycarbonate membranes with pore radii ranging from 25 to 200 nm. We show that, when vesicles larger than the membrane pore size are extruded, the minimum attainable value of mean radius of resulting liposomes is independent of initial vesicle size as well as the number of extrusion cycles. We also show that bilayer composition significantly influences the extruded liposome size. These results provide new insights into the possible mechanisms of vesicle size reduction during extrusion process. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
- Subjects :
- Phospholipid
Gene-Expression
Biochemistry
Physiological Conditions
Lipid Vesicles
Polyethylene Glycols
law.invention
Nanopores
chemistry.chemical_compound
Electricity
Size
law
Poly(Ehtylene Glycol)
Biomembranes
Pressure
Dppc
Particle Size
Molecular Biology
Phospholipids
Unilamellar Liposomes
Filtration
Liposome
Polycarboxylate Cement
Chromatography
Membranes
Vesicle
Bilayer
Confocal Laser Scanning Microscopy
Organic Chemistry
Membranes, Artificial
Equipment Design
Cell Biology
Polydispersity
Membrane
Chemical engineering
chemistry
Correlation Spectroscopy
Tension
Liposomes
Extrusion
Particle size
Small Unilamellar Vesicle
Subjects
Details
- Language :
- English
- ISSN :
- 23813652
- Database :
- OpenAIRE
- Journal :
- IndraStra Global
- Accession number :
- edsair.doi.dedup.....e6eaa7197d0b69086ff1aec3717a8959
- Full Text :
- https://doi.org/10.1016/j.chemphyslip.2013.01.003