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In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles
- Source :
- Journal of Biomedical Science, Journal of Biomedical Science, BioMed Central, 2010, 17 (1), pp.51. ⟨10.1186/1423-0127-17-51⟩, Journal of Biomedical Science, Vol 17, Iss 1, p 51 (2010)
- Publication Year :
- 2010
- Publisher :
- HAL CCSD, 2010.
-
Abstract
- Background RNA interference (RNAi) is a powerful tool to silence gene expression post-transcriptionally. Delivering sequences of RNAi in vivo remains a problem. The aim of this study was to use JC virus (JCV) virus-like particles (VLPs) as a vector for delivering RNAi in silencing the cytokine gene of IL-10. Methods JCV VLPs were generated by recombinant JCV VP1 protein in yeast expression system. DNA fragment containing IL-10 shRNA was packaged into VLPs by osmotic shock. Results In RAW 264.7 cells, IL-10 shRNA was found to reduce IL-10 expression by 85 to 89%, as compared with VLPs alone. IL-10 shRNA did not cross-react with TNF-alpha mRNA or influence the expression of TNF-alpha. In BALB/c mice IL-10 shRNA could reduce 95% of IL-10 secretion. Surprisingly, it also down regulated TNF-alpha expression. Conclusions We show for the first time that JCV VLPs empty capsids are competent vectors to deliver RNAi and are nontoxic to cells, suggesting that JCV VLPs is an efficient agent to deliver RNAi in both murine macrophage cells and BALB/c mice. This system provides an efficient means for delivering the RNAi for gene therapy purposes.
- Subjects :
- MESH: Interleukin-10
Endocrinology, Diabetes and Metabolism
viruses
[SDV]Life Sciences [q-bio]
Clinical Biochemistry
JC virus
lcsh:Medicine
Gene Expression
MESH: Base Sequence
medicine.disease_cause
Mice
chemistry.chemical_compound
0302 clinical medicine
RNA interference
MESH: Genetic Vectors
Gene expression
MESH: RNA, Small Interfering
Pharmacology (medical)
MESH: Animals
RNA, Small Interfering
MESH: Capsid Proteins
Mice, Inbred BALB C
0303 health sciences
virus diseases
General Medicine
JC Virus
Interleukin-10
[SDV] Life Sciences [q-bio]
RNA silencing
MESH: Virion
Female
RNA Interference
DNA, Complementary
MESH: Gene Expression
Genetic Vectors
MESH: RNA Interference
MESH: Mice, Inbred BALB C
MESH: JC Virus
In Vitro Techniques
Biology
Cell Line
03 medical and health sciences
In vivo
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
medicine
Animals
Gene silencing
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Molecular Biology
MESH: Mice
030304 developmental biology
Biochemistry, medical
Base Sequence
Tumor Necrosis Factor-alpha
Research
lcsh:R
Biochemistry (medical)
Virion
RNA
Cell Biology
MESH: DNA, Complementary
Virology
MESH: Cell Line
chemistry
MESH: Tumor Necrosis Factor-alpha
Capsid Proteins
MESH: Female
DNA
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 10217770 and 14230127
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Science, Journal of Biomedical Science, BioMed Central, 2010, 17 (1), pp.51. ⟨10.1186/1423-0127-17-51⟩, Journal of Biomedical Science, Vol 17, Iss 1, p 51 (2010)
- Accession number :
- edsair.doi.dedup.....e6fdaadf9d87f2642a2ed6db98a312ef