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A distinct inner nuclear membrane proteome in Saccharomyces cerevisiae gametes
- Source :
- G3: Genes|Genomes|Genetics, G3: Genes, Genomes, Genetics, Vol 11, Iss 12 (2021)
- Publication Year :
- 2021
- Publisher :
- Oxford University Press (OUP), 2021.
-
Abstract
- The inner nuclear membrane (INM) proteome regulates gene expression, chromatin organization, and nuclear transport; however, it is poorly understood how changes in INM protein composition contribute to developmentally regulated processes, such as gametogenesis. We conducted a screen to determine how the INM proteome differs between mitotic cells and gametes. In addition, we used a strategy that allowed us to determine if spores synthesize their INM proteins de novo, rather than inheriting their INM proteins from the parental cell. This screen used a split-GFP complementation system, where we were able to compare the distribution of all C-terminally tagged transmembrane proteins in Saccharomyces cerevisiae in gametes to that of mitotic cells. Gametes contain a distinct INM proteome needed to complete gamete formation, including expression of genes linked to cell wall biosynthesis, lipid biosynthetic and metabolic pathways, protein degradation, and unknown functions. Based on the inheritance pattern, INM components are made de novo in the gametes. Whereas mitotic cells show a strong preference for proteins with small extraluminal domains, gametes do not exhibit this size preference likely due to the changes in the nuclear permeability barrier during gametogenesis. Taken together, our data provide evidence for INM changes during gametogenesis and shed light on mechanisms used to shape the INM proteome of spores.
- Subjects :
- AcademicSubjects/SCI01140
sporulation
Saccharomyces cerevisiae Proteins
Proteome
AcademicSubjects/SCI00010
Nuclear Envelope
Saccharomyces cerevisiae
QH426-470
Protein degradation
AcademicSubjects/SCI01180
Genetics
Inner membrane
budding yeast
Molecular Biology
Mitosis
Genetics (clinical)
Gametogenesis
Heh1/Heh2
biology
reticulon
Mutant Screen Report
biology.organism_classification
Transmembrane protein
Chromatin
Cell biology
Germ Cells
AcademicSubjects/SCI00960
INM
Subjects
Details
- ISSN :
- 21601836
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- G3 Genes|Genomes|Genetics
- Accession number :
- edsair.doi.dedup.....e719d1b3b7f02535f14c383624de9701