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The Crimean-Congo Hemorrhagic Fever Virus NSm Protein Is Dispensable for Growth In Vitro and Disease in Ifnar-/- Mice
- Source :
- Microorganisms, Microorganisms, Vol 8, Iss 775, p 775 (2020), Volume 8, Issue 5
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Crimean-Congo hemorrhagic fever virus (CCHFV) is a tri-segmented, tick-borne nairovirus that causes disease of ranging severity in humans. The CCHFV M segment encodes a complex glycoprotein precursor (GPC) that undergoes extensive endoproteolytic cleavage, giving rise to two structural proteins (Gn and Gc) required for virus attachment and entry, and to multiple non-structural proteins (NSm, GP160, GP85, and GP38). The functions of these non-structural proteins remain largely unclear. Here, we investigate the role of NSm during infection by generating a recombinant CCHFV lacking the complete NSm domain (10200∆NSm) and observing CCHFV ∆NSm replication in cell lines and pathogenicity in Ifnar-/- mice. Our data demonstrate that the NSm domain is dispensable for viral replication in vitro, and, despite the delayed onset of clinical signs, CCHFV lacking this domain caused severe or lethal disease in infected mice.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Crimean–Congo hemorrhagic fever
030106 microbiology
Microbiology
Article
Virus
reporter virus
law.invention
reverse genetics
03 medical and health sciences
Ifnar knockout mouse
law
Virology
CCHF
medicine
lcsh:QH301-705.5
chemistry.chemical_classification
Nairovirus
biology
medicine.disease
biology.organism_classification
Reverse genetics
Ifnar
fluorescent protein reporter
030104 developmental biology
lcsh:Biology (General)
Viral replication
chemistry
ZsGreen1
Recombinant DNA
Crimean-Congo hemorrhagic fever
in vivo imaging
non-structural protein
NSm
Glycoprotein
Crimean Congo hemorrhagic fever virus
Subjects
Details
- ISSN :
- 20762607
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....e71d243b9fc1ae420ac4da256ced04e5