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Molecular cloning and sequencing ofinfC, the gene encoding translation initiation factor IF3, from four enterobacterial species
- Source :
- FEMS Microbiology Letters. 112:211-216
- Publication Year :
- 1993
- Publisher :
- Oxford University Press (OUP), 1993.
-
Abstract
- Translation initiation factor IF3 plays a crucial role in initiation of protein synthesis in bacteria. In order to elucidate the IF3 structural elements required for these functions, the evolutionary conservation of IF3 and its gene, infC, was investigated. Homologous infC sequences from Salmonella typhimurium, Klebsiella pneumoniae, Serratia marcescens and Proteus vulgaris were amplified by the polymerase chain reaction and sequenced. Analysis of these sequences, as well as that from Bacillus stearothermophilus, revealed several regions (e.g. residues 62-73 and 173-177) of absolute sequence conservation, suggesting an important role for these regions in IF3 function.
- Subjects :
- DNA, Bacterial
Salmonella typhimurium
Molecular Sequence Data
Prokaryotic Initiation Factor-3
Biology
Molecular cloning
Microbiology
Homology (biology)
law.invention
Conserved sequence
chemistry.chemical_compound
Eukaryotic translation
Bacterial Proteins
Enterobacteriaceae
Species Specificity
Peptide Initiation Factors
law
Escherichia coli
Genetics
Proteus vulgaris
Amino Acid Sequence
Cloning, Molecular
Molecular Biology
Gene
Serratia marcescens
Polymerase chain reaction
Base Sequence
Sequence Homology, Amino Acid
Nucleic acid sequence
Klebsiella pneumoniae
chemistry
Genes, Bacterial
bacteria
DNA
Subjects
Details
- ISSN :
- 15746968 and 03781097
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Letters
- Accession number :
- edsair.doi.dedup.....e71fac0df88a9c21a9f2086e4c0c86a2