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The Gene Expression Profiling in Murine Cortical Cells Undergoing Programmed Cell Death (PCD) Induced by Serum Deprivation
- Source :
- BMB Reports. 40:277-285
- Publication Year :
- 2007
- Publisher :
- Korean Society for Biochemistry and Molecular Biology - BMB Reports, 2007.
-
Abstract
- PCD (programmed cell death) is important mechanism for development, homeostasis and disease. To analyze the gene expression pattern in brain cells undergoing PCD in response to serum deprivation, we analyzed the cDNA microarray consisting of 2,300 genes and 7 housekeeping genes of cortical cells derived from mouse embryonic brain. Cortical cells were induced apoptosis by serum deprivation for 8 hours. We identified 69 up-regulated genes and 21 down-regulated genes in apoptotic cells. Based on the cDNA microarray data four genes were selected and analyzed by RT-PCR and northern blotting. To characterize the role of UNC-51-like kinase (ULK2) gene in PCD, we investigated cell death effect by ULK2. And we examined expression of several genes that related with PCD. Especially GAPDH was increased by ULK2. Theses findings indicated that ULK2 is involved in apoptosis through p53 pathway.
- Subjects :
- Serum
Programmed cell death
Down-Regulation
Apoptosis
Protein Serine-Threonine Kinases
Biology
Biochemistry
Mice
Complementary DNA
Gene expression
Animals
RNA, Messenger
Northern blot
Molecular Biology
Gene
Cells, Cultured
Cerebral Cortex
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Reproducibility of Results
General Medicine
Molecular biology
Up-Regulation
Cell biology
Housekeeping gene
Gene expression profiling
Subjects
Details
- ISSN :
- 19766696
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....e726462e05d7d9d269b6d342282d4c78