Back to Search Start Over

Small RNA changes en route to distinct cellular states of induced pluripotency

Authors :
Clancy, Jennifer L
Patel, Hardip R
Hussein, Samer M I
Tonge, Peter D
Cloonan, Nicole
Corso, Andrew J
Li, Mira
Lee, Dong-Sung
Shin, Jong-Yeon
Wong, Justin J L
Bailey, Charles G
Benevento, Marco
Munoz, Javier
Chuah, Aaron
Wood, David
Rasko, John E J
Heck, Albert J R
Grimmond, Sean M
Rogers, Ian M
Seo, Jeong-Sun
Wells, Christine A
Puri, Mira C
Nagy, Andras
Preiss, Thomas
Sub Biomol.Mass Spectrometry & Proteom.
Sub Biomol.Mass Spect. and Proteomics
Biomolecular Mass Spectrometry and Proteomics
Molecular Pharmacy
Sub Biomol.Mass Spectrometry & Proteom.
Sub Biomol.Mass Spect. and Proteomics
Biomolecular Mass Spectrometry and Proteomics
Molecular Pharmacy
Source :
Nature Communications [E], 5. Nature Publishing Group
Publication Year :
2014

Abstract

MicroRNAs (miRNAs) are critical to somatic cell reprogramming into induced pluripotent stem cells (iPSCs), however, exactly how miRNA expression changes support the transition to pluripotency requires further investigation. Here we use a murine secondary reprogramming system to sample cellular trajectories towards iPSCs or a novel pluripotent 'F-class' state and perform small RNA sequencing. We detect sweeping changes in an early and a late wave, revealing that distinct miRNA milieus characterize alternate states of pluripotency. miRNA isoform expression is common but surprisingly varies little between cell states. Referencing other omic data sets generated in parallel, we find that miRNA expression is changed through transcriptional and post-transcriptional mechanisms. miRNA transcription is commonly regulated by dynamic histone modification, while DNA methylation/demethylation consolidates these changes at multiple loci. Importantly, our results suggest that a novel subset of distinctly expressed miRNAs supports pluripotency in the F-class state, substituting for miRNAs that serve such roles in iPSCs.

Details

ISSN :
20411723
Volume :
5
Database :
OpenAIRE
Journal :
Nature communications
Accession number :
edsair.doi.dedup.....e73b8f9735a284ca101da625b7adaa38