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IL-10-producing B cells regulate Th1/Th17-cell immune responses inPneumocystispneumonia
- Source :
- American Journal of Physiology-Lung Cellular and Molecular Physiology. 316:L291-L301
- Publication Year :
- 2019
- Publisher :
- American Physiological Society, 2019.
-
Abstract
- Pneumocystis pneumonia (PCP) is a common opportunistic infectious disease that is prevalent in immunosuppressed hosts. Accumulating evidence shows that B cells play an important role in infectious diseases. In the present study, the immune regulatory role of mature B cells in host defense to Pneumocystis was evaluated. Pneumocystis infection resulted in a decrease in B cells in patients and mice, and the Pneumocystis burden in B cell-deficient mice also progressively increased from weeks 1 to 7 after infection. The clearance of Pneumocystis was delayed in B cell-activating factor receptor (BAFF-R)-deficient mice (BAFF-R−/−mice), which had few B cells and Pneumocystis-specific IgG and IgM antibodies, compared with clearance in wild-type (WT) mice. There were fewer effector CD4+T cells and higher percentages of T helper (Th)1/Th17 cells in BAFF-R−/−mice than in WT mice. Adoptive transfer of naive B cells, mRNA sequencing, and IL-1β neutralization experiments indicated that IL-1β is a likely determinant of the IL-10-producing B cell-mediated suppression of Th1/Th17-cell immune responses in BAFF-R−/−PCP mice. Our data indicated that B cells play a vital role in the regulation of Th cells in response to Pneumocystis infection.
- Subjects :
- Adult
Male
0301 basic medicine
Pulmonary and Respiratory Medicine
Physiology
Interleukin-1beta
Cell
Pneumocystis pneumonia
Mice
03 medical and health sciences
Th2 Cells
0302 clinical medicine
Immune system
Physiology (medical)
medicine
Animals
Humans
Th1 th17
Antibodies, Fungal
Mice, Knockout
B-Lymphocytes
Pneumocystis
business.industry
Pneumonia, Pneumocystis
Cell Biology
Th1 Cells
Immunosuppressed Hosts
medicine.disease
Interleukin-10
Interleukin 10
Pneumonia
030104 developmental biology
medicine.anatomical_structure
Immunoglobulin M
030228 respiratory system
Immunoglobulin G
Opportunistic infectious disease
Immunology
Female
business
B-Cell Activation Factor Receptor
Subjects
Details
- ISSN :
- 15221504 and 10400605
- Volume :
- 316
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Lung Cellular and Molecular Physiology
- Accession number :
- edsair.doi.dedup.....e73e8df140492297a9a8b3351e91ce9c