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Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry

Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry

Authors :
Li Wu
Alicia M. Janas
Chunsheng Dong
Jian-Hua Wang
Source :
Virology. 375:442-451
Publication Year :
2008
Publisher :
Elsevier BV, 2008.

Abstract

Human immunodeficiency virus type 1 (HIV-1) enters dendritic cells (DCs) through endocytosis and viral receptor-mediated fusion. Although endocytosis-mediated HIV-1 entry can generate productive infection in certain cell types, including human monocyte-derived macrophages, productive HIV-1 infection in DCs appears to be dependent on fusion-mediated viral entry. It remains to be defined whether endocytosed HIV-1 in DCs can initiate productive infection. Using HIV-1 infection and cellular fractionation assays to measure productive viral infection and entry, here we show that HIV-1 enters monocyte-derived DCs predominately through endocytosis; however, endocytosed HIV-1 cannot initiate productive HIV-1 infection in DCs. In contrast, productive HIV-1 infection in DCs requires fusion-mediated viral entry. Together, these results provide functional evidence in understanding HIV-1 cis-infection of DCs, suggesting that different pathways of HIV-1 entry into DCs determine the outcome of viral infection.

Details

ISSN :
00426822
Volume :
375
Database :
OpenAIRE
Journal :
Virology
Accession number :
edsair.doi.dedup.....e7436bf9b0d4f36d981b275ef03f02b4
Full Text :
https://doi.org/10.1016/j.virol.2008.01.044