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Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry
Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry
- Source :
- Virology. 375:442-451
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- Human immunodeficiency virus type 1 (HIV-1) enters dendritic cells (DCs) through endocytosis and viral receptor-mediated fusion. Although endocytosis-mediated HIV-1 entry can generate productive infection in certain cell types, including human monocyte-derived macrophages, productive HIV-1 infection in DCs appears to be dependent on fusion-mediated viral entry. It remains to be defined whether endocytosed HIV-1 in DCs can initiate productive infection. Using HIV-1 infection and cellular fractionation assays to measure productive viral infection and entry, here we show that HIV-1 enters monocyte-derived DCs predominately through endocytosis; however, endocytosed HIV-1 cannot initiate productive HIV-1 infection in DCs. In contrast, productive HIV-1 infection in DCs requires fusion-mediated viral entry. Together, these results provide functional evidence in understanding HIV-1 cis-infection of DCs, suggesting that different pathways of HIV-1 entry into DCs determine the outcome of viral infection.
- Subjects :
- Cell type
Human immunodeficiency virus (HIV)
HIV Infections
Biology
Cell Fractionation
Endocytosis
medicine.disease_cause
Membrane Fusion
Dendritic cells
Article
03 medical and health sciences
Viral entry
Virology
medicine
Humans
Cellular fractionation
Fusion
Receptor
Cells, Cultured
030304 developmental biology
0303 health sciences
030302 biochemistry & molecular biology
virus diseases
Lipid bilayer fusion
Virus Internalization
3. Good health
Immunology
HIV-1
Entry pathway
Cell fractionation
Infection
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 375
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....e7436bf9b0d4f36d981b275ef03f02b4
- Full Text :
- https://doi.org/10.1016/j.virol.2008.01.044