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Crystal structure of the 14-subunit RNA polymerase I
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2013
- Publisher :
- Springer Nature, 2013.
-
Abstract
- 16 p.-5 fig.-8 extend. dat. fig.-1 extend. dat. tab.<br />Protein biosynthesis depends on the availability of ribosomes, which in turn relies on ribosomal RNA production. In eukaryotes, this process is carried out by RNA polymerase I (Pol I), a 14-subunit enzyme, the activity of which is a major determinant of cell growth. Here we present the crystal structure of Pol I from Saccharomyces cerevisiae at 3.0 Å resolution. The Pol I structure shows a compact core with a wide DNA-binding cleft and a tightly anchored stalk. An extended loop mimics the DNA backbone in the cleft and may be involved in regulating Pol I transcription. Subunit A12.2 extends from the A190 jaw to the active site and inserts a transcription elongation factor TFIIS-like zinc ribbon into the nucleotide triphosphate entry pore, providing insight into the role of A12.2 in RNA cleavage and Pol I insensitivity to α-amanitin. The A49–A34.5 heterodimer embraces subunit A135 through extended arms, thereby contacting and potentially regulating subunit A12.2.<br />M.M.-M. and U.J.R. were supported by EMBO Long-Term fellowships, M.M.-M. by the Marie-Curie fellowship (FP7-PEOPLE-2011-IEF 301002), N.M.I.T. by a Fundación Futuro fellowship, F.M.R. by an ESF/CSIC funded JAE-DOC contract and T.G. by the Volkswagen Stiftung via the Niedersachsenprofessur of Prof. G. M. Sheldrick. This work was also partly funded by grant BFU2010-16336 of the Spanish Ministry of Science.
- Subjects :
- DNA polymerase
5.8S ribosomal RNA
RNA-dependent RNA polymerase
Ribosome
Conformational flexibility
Electron-microscopy
RNA polymerase III
03 medical and health sciences
0302 clinical medicine
Subunits
Architecture
RNA polymerase I
Transcriptional regulation
Elongation complex
030304 developmental biology
0303 health sciences
Multidisciplinary
biology
TFIIF
Transcription initiation
Processivity
Biochemistry
Phase information
biology.protein
030217 neurology & neurosurgery
Cross-linking
Model
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Accession number :
- edsair.doi.dedup.....e7479e73fdd4e63822285f1134d95b3e