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Attenuation of Intestinal Epithelial Cell Migration During Cryptosporidium parvum Infection Involves Parasite Cdg7_FLc_1030 RNA-Mediated Induction and Release of Dickkopf-1
- Source :
- The Journal of Infectious Diseases. 218:1336-1347
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Intestinal infection by Cryptosporidium is known to cause epithelial cell migration disorder but the underlying mechanisms are unclear. Previous studies demonstrated that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using multiple models of intestinal cryptosporidiosis, we report here that C. parvum infection induces expression and release of the dickkopf protein 1 (Dkk1) from intestinal epithelial cells. Delivery of parasite Cdg7_FLc_1030 RNA to intestinal epithelial cells triggers transactivation of host Dkk1 gene during C. parvum infection. Release of Dkk1 is involved in C. parvum-induced inhibition of cell migration of epithelial cells, including noninfected bystander cells. Moreover, Dkk1-mediated suppression of host cell migration during C. parvum infection involves inhibition of Cdc42/Par6 signaling. Our data support the hypothesis that attenuation of intestinal epithelial cell migration during Cryptosporidium infection involves parasite Cdg7_FLc_1030 RNA-mediated induction and release of Dkk1 from infected cells.
- Subjects :
- Transcriptional Activation
musculoskeletal diseases
0301 basic medicine
030106 microbiology
CDC42
Biology
Epithelial cell migration
Cell Line
Microbiology
Mice
Major Articles and Brief Reports
03 medical and health sciences
Transactivation
parasitic diseases
Intestinal epithelial cell migration
Animals
Humans
Immunology and Allergy
Intestinal Mucosa
Cryptosporidium parvum
RNA
Epithelial Cells
Cell migration
biology.organism_classification
030104 developmental biology
Infectious Diseases
Gene Expression Regulation
Cell culture
Intercellular Signaling Peptides and Proteins
RNA, Protozoan
Subjects
Details
- ISSN :
- 15376613 and 00221899
- Volume :
- 218
- Database :
- OpenAIRE
- Journal :
- The Journal of Infectious Diseases
- Accession number :
- edsair.doi.dedup.....e766eb5f9bf6929289faa75cb35099bc