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Analysis of genes expression of Spodoptera exigua larvae upon AcMNPV infection
- Source :
- PLoS ONE, Vol 7, Iss 7, p e42462 (2012), PLOS ONE(7): 7, PLoS ONE
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- Background: The impact of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) infection on host gene expression in Spodoptera exigua 4th instar larvae was investigated through the use of 454 sequencing-based RNA-seq of cDNA libraries developed from insects challenged with active AcMNPV or heat-inactivated AcMNPV. Methodology/Principal Findings: By comparing the two cDNA libraries, we show that 201 host genes are significantly up-regulated and 234 genes are significantly down-regulated by active AcMNPV infection. Down-regulated host genes included genes encoding antimicrobial peptides, namely three gloverin isoforms and an attacin, indicating that the viral infection actively repressed the expression of a portion of the host immune gene repertoire. Another interesting group of down-regulated host genes included genes encoding two juvenile hormone binding proteins and a hexamerin, all of which are involved in juvenile hormone regulation. The expression of these genes was enhanced by the topical application of Juvenile Hormone III (JHIII) in the insects challenged with heat-inactivated AcMNPV. However, infection with the active virus strongly suppresses the expression of these three genes, regardless of the absence or presence of JHIII. Conclusions/Significance: Using RNA-seq, we have identified groups of immune-regulated and juvenile hormone-regulated genes that are suppressed by infection with active AcMNPV. This information and further studies on the regulation of host gene expression by AcMNPV will provide the tools needed to enhance the utility of the virus as an effective protein expression system and as an insecticide.
- Subjects :
- animal structures
DNA, Complementary
viruses
lcsh:Medicine
Spodoptera
Microbiology
Virology
Microbial Control
Exigua
Gene expression
Animals
Pesticides
lcsh:Science
Gene
Biology
Multidisciplinary
biology
cDNA library
Gene Expression Profiling
Host Cells
fungi
lcsh:R
Agriculture
Genomics
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Nucleopolyhedroviruses
Functional Genomics
Gene expression profiling
Autographa californica
Larva
Instar
lcsh:Q
Pest Control
Transcriptome
Agrochemicals
Zoology
Entomology
Viral Transmission and Infection
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....e787150d5376dd2e125bbe2d6b135472