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HuR and GRSF1 modulate the nuclear export and mitochondrial localization of the lncRNA RMRP
- Source :
- Genes & Development. 30:1224-1239
- Publication Year :
- 2016
- Publisher :
- Cold Spring Harbor Laboratory, 2016.
-
Abstract
- Some mitochondrial long noncoding RNAs (lncRNAs) are encoded by nuclear DNA, but the mechanisms that mediate their transport to mitochondria are poorly characterized. Using affinity RNA pull-down followed by mass spectrometry analysis, we found two RNA-binding proteins (RBPs), HuR (human antigen R) and GRSF1 (G-rich RNA sequence-binding factor 1), that associated with the nuclear DNA-encoded lncRNA RMRP and mobilized it to mitochondria. In cultured human cells, HuR bound RMRP in the nucleus and mediated its CRM1 (chromosome region maintenance 1)-dependent export to the cytosol. After RMRP was imported into mitochondria, GRSF1 bound RMRP and increased its abundance in the matrix. Loss of GRSF1 lowered the mitochondrial levels of RMRP, in turn suppressing oxygen consumption rates and modestly reducing mitochondrial DNA replication priming. Our findings indicate that RBPs HuR and GRSF1 govern the cytoplasmic and mitochondrial localization of the lncRNA RMRP, which is encoded by nuclear DNA but has key functions in mitochondria.
- Subjects :
- 0301 basic medicine
Active Transport, Cell Nucleus
RNA-binding protein
Biology
Mitochondrion
Poly(A)-Binding Proteins
ELAV-Like Protein 1
03 medical and health sciences
Genetics
Humans
Nuclear export signal
Cell Nucleus
RNA
Molecular biology
Mitochondria
Cell biology
Nuclear DNA
Protein Transport
Cytosol
HEK293 Cells
030104 developmental biology
Cytoplasm
RNA, Long Noncoding
HeLa Cells
Protein Binding
Research Paper
Developmental Biology
Mitochondrial DNA replication
Subjects
Details
- ISSN :
- 15495477 and 08909369
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Genes & Development
- Accession number :
- edsair.doi.dedup.....e78bd663c13ea6cbdb9a4ad4798c20ab