Back to Search
Start Over
SUPT4H1 Depletion Leads to a Global Reduction in RNA
- Source :
- Cell reports. 26(1)
- Publication Year :
- 2018
-
Abstract
- Summary SUPT4H1 is a transcription elongation factor that makes up part of the RNA polymerase II complex. Recent studies propose a selective role for SUPT4H1 in the transcription of repeat-containing DNA, the translated products of which contribute to neurodegenerative disorders such as C9orf72-amyotrophic lateral sclerosis. To investigate the potential of SUPT4H1 as a therapeutic target in repeat-associated neurodegeneration, we depleted SUPT4H1 by RNA interference to inhibit the function of the SUPT4H1/SUPT5H transcription elongation complex. Depletion of SUPT4H1 leads to a global reduction in all cellular RNA, highlighting the significant challenges that are associated with targeting this molecule for the treatment of human disease. Any requirement of SUPT4H1 for transcription of specific transcripts should be interpreted in the context of global modulatory effects on the transcriptome.
- Subjects :
- Transcription elongation
Chromosomal Proteins, Non-Histone
RNA polymerase II complex
General Biochemistry, Genetics and Molecular Biology
Transcriptome
chemistry.chemical_compound
Human disease
Transcription (biology)
medicine
Humans
RNA, Messenger
Neurodegeneration
RNA
medicine.disease
Cell biology
Repressor Proteins
HEK293 Cells
chemistry
A549 Cells
Gene Knockdown Techniques
RNA Interference
Transcriptional Elongation Factors
Ribosomes
DNA
HeLa Cells
Subjects
Details
- ISSN :
- 22111247
- Volume :
- 26
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cell reports
- Accession number :
- edsair.doi.dedup.....e7b03666895b948d2895b81d3859653f