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Enhancement of HIFU ablation by sonosensitizer-loading liquid fluorocarbon nanoparticles with pre-targeting in a mouse model
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-18 (2019)
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- High intensity focused ultrasound (HIFU) is a noninvasive thermal ablation technique for the treatment of benign and malignant solid masses. To improve the efficacy of HIFU ablation, we developed poly (lactide-co-glycolide) (PLGA) nanoparticles encapsulating perfluoropentane (PFP) and hematoporphyrin monomethyl ether (HMME) as synergistic agents (HMME+PFP/PLGA). Two-step biotin-avidin pre-targeting technique was applied for the HIFU ablation. We further modified the nanoparticles with streptavidin (HMME+PFP/PLGA-SA). HMME+PFP/PLGA-SA were highly dispersed with spherical morphology (477.8 ± 81.8 nm in diameter). The encapsulation efficiency of HMME and PFP were 46.6 ± 3.3% and 40.1 ± 2.6%, respectively. The binding efficiency of nanoparticles to streptavidin was 95.5 ± 2.5%. The targeting ability of the HMME+PFP/PLGA-SA nanoparticles was tested by parallel plate flow chamber in vitro. In the pre-targeting group (HMME+PFP/PLGA-SA), a large number of nanoparticles bound to the peripheral and surface of the cell. In the HIFU ablation experiment in vivo, compared with the other groups, the largest gray-scale changes and coagulation necrosis areas were observed in the pre-targeting (HMME+PFP/PLGA-SA) group, with the lowest energy efficiency factor value. Moreover, the microvessel density and proliferation index declined, while the apoptotic index increased, in the tumor tissue surrounding the coagulation necrosis area in the pre-targeting group. Meanwhile, the survival time of the tumor-bearing nude mice in the pre-targeting group was significantly longer than that in the HIFU treatment group. These results suggest that HMME+PFP/PLGA-SA have high potential to act as synergistic agents in HIFU ablation.
- Subjects :
- 0301 basic medicine
Streptavidin
Proliferation index
Parallel-plate flow chamber
medicine.medical_treatment
lcsh:Medicine
Mice, Nude
Nanoparticle
Apoptosis
Breast Neoplasms
Article
Mice
03 medical and health sciences
chemistry.chemical_compound
Targeted therapies
0302 clinical medicine
In vivo
Tumor Cells, Cultured
medicine
Animals
Humans
lcsh:Science
Cell Proliferation
Fluorocarbons
Mice, Inbred BALB C
Multidisciplinary
lcsh:R
technology, industry, and agriculture
Antibodies, Monoclonal
Combined Modality Therapy
Vascular Endothelial Growth Factor Receptor-2
Xenograft Model Antitumor Assays
High-intensity focused ultrasound
Disease Models, Animal
Hematoporphyrins
PLGA
030104 developmental biology
Coagulative necrosis
chemistry
Outcomes research
High-Intensity Focused Ultrasound Ablation
Nanoparticles
lcsh:Q
Female
030217 neurology & neurosurgery
Biomedical engineering
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....e7c9ad42ca71cd0c9ab5ec7624285b88