Back to Search
Start Over
Diabetes Induces Abnormal Ovarian Function via Triggering Apoptosis of Granulosa Cells and Suppressing Ovarian Angiogenesis
- Source :
- International Journal of Biological Sciences
- Publication Year :
- 2017
- Publisher :
- Ivyspring International Publisher, 2017.
-
Abstract
- Diabetes triggers abnormal ovarian follicular development and consequently leads to infertility. Here, we established a type 2 diabetes mouse model by feeding with high fat diet (HFD) for 15/20 weeks and assessed the effect of diabetes on follicular development and ovarian angiogenesis. After fed with HFD for 15 weeks, mice had the characteristics of type 2 diabetes, which was much more serious after 20 weeks on HFD. After 20 weeks on HFD, the mice had shown abnormal ovarian morphology with hyaline appearance, much less blood vessel, follicular development arrest and less of granulosa cells (GCs) in mature follicles, but not in ovaries from 15 weeks on HFD. Elevated makers of DNA damage, ER stress and apoptosis of GCs were observed in ovaries from HFD for 20 weeks. Additionally, diabetes significantly suppressed ovarian angiogenesis with the evidence of down-regulation of CD31 via inhibiting HIF1α-VEGF signaling pathway in time-dependent. We concluded that diabetes triggers abnormal ovarian function via inducing GCs apoptosis and suppressing ovarian angiogenesis.
- Subjects :
- granulosa cells (GCs)
0301 basic medicine
CD31
endocrine system
medicine.medical_specialty
Angiogenesis
Ovary
Type 2 diabetes
Applied Microbiology and Biotechnology
angiogenesis
Mice
03 medical and health sciences
Ovarian Follicle
Diabetes mellitus
Internal medicine
Follicular phase
Animals
Medicine
Ovarian follicle
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Granulosa Cells
diabetes
business.industry
apoptosis
Cell Biology
medicine.disease
Mice, Inbred C57BL
030104 developmental biology
Endocrinology
medicine.anatomical_structure
DNA damage
Apoptosis
Female
business
hormones, hormone substitutes, and hormone antagonists
Research Paper
DNA Damage
Developmental Biology
Subjects
Details
- ISSN :
- 14492288
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....e7eb43d94b5dc0150ff9985aea648f36