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Comparison of epsilon- and delta-class glutathioneS-transferases: the crystal structures of the glutathioneS-transferases DmGSTE6 and DmGSTE7 fromDrosophila melanogaster
- Source :
- Acta Crystallographica Section D Biological Crystallography. 71:2089-2098
- Publication Year :
- 2015
- Publisher :
- International Union of Crystallography (IUCr), 2015.
-
Abstract
- Cytosolic glutathione transferases (GSTs) comprise a large family of enzymes with canonical structures that diverge functionally and structurally among mammals, invertebrates and plants. Whereas mammalian GSTs have been characterized extensively with regard to their structure and function, invertebrate GSTs remain relatively unstudied. The invertebrate GSTs do, however, represent potentially important drug targets for infectious diseases and agricultural applications. In addition, it is essential to fully understand the structure and function of invertebrate GSTs, which play important roles in basic biological processes. Invertebrates harbor delta- and epsilon-class GSTs, which are not found in other organisms.Drosophila melanogasterGSTs (DmGSTs) are likely to contribute to detoxication or antioxidative stress during development, but they have not been fully characterized. Here, the structures of two epsilon-class GSTs fromDrosophila, DmGSTE6 and DmGSTE7, are reported at 2.1 and 1.5 Å resolution, respectively, and are compared with other GSTs to identify structural features that might correlate with their biological functions. The structures of DmGSTE6 and DmGSTE7 are remarkably similar; the structures do not reveal obvious sources of the minor functional differences that have been observed. The main structural difference between the epsilon- and delta-class GSTs is the longer helix (A8) at the C-termini of the epsilon-class enzymes.
- Subjects :
- Models, Molecular
Protein Conformation
Molecular Sequence Data
Sequence alignment
Protein structure
Structural Biology
Animals
Drosophila Proteins
Transferase
Amino Acid Sequence
Peptide sequence
Glutathione Transferase
Genetics
Binding Sites
biology
Protein Stability
Temperature
General Medicine
biology.organism_classification
Research Papers
Glutathione
Drosophila melanogaster
Glutathione S-transferase
Biochemistry
biology.protein
Sequence Alignment
Glutathione binding
Drosophila Protein
Subjects
Details
- ISSN :
- 13990047
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Acta Crystallographica Section D Biological Crystallography
- Accession number :
- edsair.doi.dedup.....e7efefa26d5927b86e9a4cbc432a0368