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Insufficient activity of MAPK pathway is a key monitor of Kidney-Yang Deficiency Syndrome
- Source :
- Journal of alternative and complementary medicine (New York, N.Y.). 15(6)
- Publication Year :
- 2009
-
Abstract
- To explore the genetic characteristics and molecular regulator of Kidney-Yang Deficiency Syndrome (KDS).A typical KDS family was collected using a questionnaire of cold feeling and a 40-item scoring table of KDS based on Traditional Chinese Medicine (TCM), by single-blind method repeated annually over three years. Their transcriptomes were assayed by microarray and validated by RT-PCR and ELISA. Simultaneously, 10 healthy volunteers were recruited as controls and the same protocols were performed.This typical KDS family has 35 members, of whom 11 were evaluated as having severe KDS and 6 as having common KDS. Results of the cDNA microarray revealed that there were 420 genes/expressed sequence tags differentially expressed in KDS transcriptomes, indicating a global functional impairment in the mass-energy-information carrying network of KDS patients, involving energy metabolism, signal transduction, development, cell cycle, and immunity. Pathway analysis by gene set enrichment assay (GSEA) and other tools demonstrated that mitogenic activated protein kinase (MAPK) is among the most insufficiently activated pathways, while the oxidative phosphorylation and glycolysis/gluconeogenesis pathways, the two main pathways relevant to ATP synthesis, were among the most excessively activated pathways in KDS patients. Results of RT-PCR and ELISA confirmed the status of insufficient activity of the MAPK pathway.KDS patients undergo overall attenuated functions in the mass-energy-information carrying network. The marked low level of energy output in KDS may be primarily attributed to the insufficient activity of the MAPK pathway, which may be a key monitor for the abnormal energy metabolism and other impaired activities in KDS.
- Subjects :
- China
Microarray
MAP Kinase Signaling System
Regulator
Gene Expression
Urination
Enzyme-Linked Immunosorbent Assay
Computational biology
Oxidative Phosphorylation
Body Temperature
Transcriptome
Adenosine Triphosphate
Complementary DNA
Gene expression
Medicine
Humans
Single-Blind Method
Medicine, Chinese Traditional
Extracellular Signal-Regulated MAP Kinases
Gene
Oligonucleotide Array Sequence Analysis
Mitogen-Activated Protein Kinase 1
Expressed sequence tag
business.industry
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Gluconeogenesis
Pedigree
Gene expression profiling
Yang Deficiency
Complementary and alternative medicine
Kidney Diseases
business
Energy Metabolism
Glycolysis
Subjects
Details
- ISSN :
- 15577708
- Volume :
- 15
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of alternative and complementary medicine (New York, N.Y.)
- Accession number :
- edsair.doi.dedup.....e7f5c959374baae1292f471c8beafc7d