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Biotinylated Phosphorus Dendrimers as Control Line in Nucleic Acid Lateral Flow Tests
- Source :
- Biomacromolecules. 21:1315-1323
- Publication Year :
- 2020
- Publisher :
- American Chemical Society (ACS), 2020.
-
Abstract
- Lateral flow assays (LFA) are an affordable, easy-to-use, qualitative rapid test for clinical diagnosis in nonlaboratory environments and low-resource facilities. The control line of these tests is very important to provide a valid result, confirming that the platform operates correctly. A clear, nondiffused line is desirable. The number of colored nanoparticles that reach the control line in a positive test can be very small, and they should all be trapped efficiently by the molecules adsorbed there. In this work, we proposed the use of robust biotinylated dendrimers of two different generations as signal amplifiers in control lines of LFA, able to react with streptavidin-modified gold nanoparticles. Besides the synthesis and characterization, the analytical performance as control lines will be studied, and their response will be compared with other commercially available biotinylated molecules. Finally, the utility of the dendrimer implemented in a NALF (Nucleic Acid Lateral Flow) strip was also demonstrated for detection of the amplicons obtained by double-tagging PCR (polymerase chain reaction) for the detection of E. coli as a model of foodborne pathogen.
- Subjects :
- Dendrimers
Polymers and Plastics
Flow (psychology)
Biotin
Metal Nanoparticles
Nanoparticle
Bioengineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Biomaterials
Control line
Nucleic Acids
Dendrimer
Escherichia coli
Materials Chemistry
Gold nanoparticles
Lateral flow
Chemistry
Phosphorus
Amplicon
021001 nanoscience & nanotechnology
Combinatorial chemistry
0104 chemical sciences
Colloidal gold
Biotinylation
Nucleic acid
Streptavidin
Gold
0210 nano-technology
Subjects
Details
- ISSN :
- 15264602 and 15257797
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Biomacromolecules
- Accession number :
- edsair.doi.dedup.....e7f7c20a4a82dc426442d18edd1d9035