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Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
- Publication Year :
- 2017
-
Abstract
- The compound 29-(4-methylpiperazine)-luepol (M22), a novel derivative of lupeol has shown anti-proliferative effects against the human non-small cell lung cancer A549 cell line. M22 showed significant anti-proliferative activity at 6.80 μM and increased accumulation of G1 cells and effectively suppressed expression of the G1 arrest-related genes cyclins D1 and E1, CDK2 and CDC25A. This was further confirmed by Western blotting demonstrating decreased cyclin D1 and CDC25A protein levels. Furthermore, M22 caused induction of apoptosis that downregulated the anti-apoptotic BCL-2 gene and increased expression of BAX, CASP3 and CASP9 as well as the APAF1 gene. The effect of caspase-induced apoptosis was confirmed by an increase in reactive oxygen species (ROS), loss of mitochondrial membrane potential (MMP). Taken together, our findings indicated that M22 possessed potent anti-proliferative and apoptotic activities.
- Subjects :
- 0301 basic medicine
CDC25A
Science
Apoptosis
Piperazines
Article
03 medical and health sciences
Inhibitory Concentration 50
0302 clinical medicine
Cyclin D1
Cell Line, Tumor
Cyclin E
Humans
cdc25 Phosphatases
bcl-2-Associated X Protein
A549 cell
chemistry.chemical_classification
Regulation of gene expression
Membrane Potential, Mitochondrial
Oncogene Proteins
Reactive oxygen species
Multidisciplinary
Caspase 3
Cyclin-Dependent Kinase 2
Hep G2 Cells
Antineoplastic Agents, Phytogenic
G1 Phase Cell Cycle Checkpoints
Caspase 9
Gene Expression Regulation, Neoplastic
030104 developmental biology
Apoptotic Protease-Activating Factor 1
chemistry
Proto-Oncogene Proteins c-bcl-2
Cell culture
A549 Cells
030220 oncology & carcinogenesis
Cancer research
Medicine
Signal transduction
Pentacyclic Triterpenes
Reactive Oxygen Species
HeLa Cells
Signal Transduction
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....e80096df946fd86b58e7a6dc3f9f3bbd