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Structure of the YajR transporter suggests a transport mechanism based on the conserved motif A
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 110(36)
- Publication Year :
- 2013
-
Abstract
- The major facilitator superfamily (MFS) is the largest family of secondary active transporters and is present in all life kingdoms. Detailed structural basis of the substrate transport and energy-coupling mechanisms of these proteins remain to be elucidated. YajR is a putative proton-driven MFS transporter found in many Gram-negative bacteria. Here we report the crystal structure of Escherichia coli YajR at 3.15 Å resolution in an outward-facing conformation. In addition to having the 12 canonical transmembrane helices, the YajR structure includes a unique 65-residue C-terminal domain which is independently stable. The structure is unique in illustrating the functional role of “sequence motif A.” This highly conserved element is seen to stabilize the outward conformation of YajR and suggests a general mechanism for the conformational change between the inward and outward states of the MFS transporters.
- Subjects :
- Models, Molecular
Conformational change
Protein Conformation
Amino Acid Motifs
Molecular Sequence Data
Biology
Crystallography, X-Ray
Protein Structure, Secondary
Protein structure
Escherichia coli
Amino Acid Sequence
Peptide sequence
Multidisciplinary
Binding Sites
Sequence Homology, Amino Acid
Membrane transport protein
Escherichia coli Proteins
Membrane Transport Proteins
Biological Transport
Biological Sciences
Major facilitator superfamily
Transport protein
Protein Structure, Tertiary
Transmembrane domain
Biochemistry
Mutation
Biophysics
biology.protein
Electrophoresis, Polyacrylamide Gel
Sequence motif
Subjects
Details
- ISSN :
- 10916490
- Volume :
- 110
- Issue :
- 36
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....e8082516bad2df82f9916ca4e1f2a137