Back to Search Start Over

Exploitation of a very small peptide nucleic acid as a new inhibitor of miR-509-3p involved in the regulation of cystic fibrosis disease-gene expression

Exploitation of a very small peptide nucleic acid as a new inhibitor of miR-509-3p involved in the regulation of cystic fibrosis disease-gene expression

Authors :
Stefano D'Errico
Giuseppe De Rosa
Gennaro Piccialli
Carmine Marco Morgillo
Bruno Catalanotti
Felice Amato
Rossella Tomaiuolo
Giuseppe Castaldo
Laura Mayol
Giorgia Oliviero
Ausilia Elce
Fabrizia Nici
Nicola Borbone
Amato, Felice
Tomaiuolo, Rossella
Nici, Fabrizia
Borbone, Nicola
Ausilia, Elce
Catalanotti, Bruno
D'Errico, Stefano
Morgillo, CARMINE MARCO
DE ROSA, Giuseppe
Mayol, Laura
Piccialli, Gennaro
Oliviero, Giorgia
Castaldo, Giuseppe
Source :
BioMed Research International, BioMed Research International, Vol 2014 (2014)
Publication Year :
2013

Abstract

Computational techniques, and in particular molecular dynamics (MD) simulations, have been successfully used as a complementary technique to predict and analyse the structural behaviour of nucleic acids, including peptide nucleic acid- (PNA-) RNA hybrids. This study shows that a 7-base long PNA complementary to the seed region of miR-509-3p, one of the miRNAs involved in the posttranscriptional regulation of the CFTR disease-gene of Cystic Fibrosis, and bearing suitable functionalization at its N- and C-ends aimed at improving its resistance to nucleases and cellular uptake, is able to revert the expression of the luciferase gene containing the 3′UTR of the gene in A549 human lung cancer cells, in agreement with the MD results that pointed at the formation of a stable RNA/PNA heteroduplex notwithstanding the short sequence of the latter. The here reported results widen the interest towards the use of small PNAs as effective anti-miRNA agents.

Details

ISSN :
23146141
Volume :
2014
Database :
OpenAIRE
Journal :
BioMed research international
Accession number :
edsair.doi.dedup.....e84e6fea2f96dbdff0d32757b33001e0