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Exploitation of a very small peptide nucleic acid as a new inhibitor of miR-509-3p involved in the regulation of cystic fibrosis disease-gene expression
Exploitation of a very small peptide nucleic acid as a new inhibitor of miR-509-3p involved in the regulation of cystic fibrosis disease-gene expression
- Source :
- BioMed Research International, BioMed Research International, Vol 2014 (2014)
- Publication Year :
- 2013
-
Abstract
- Computational techniques, and in particular molecular dynamics (MD) simulations, have been successfully used as a complementary technique to predict and analyse the structural behaviour of nucleic acids, including peptide nucleic acid- (PNA-) RNA hybrids. This study shows that a 7-base long PNA complementary to the seed region of miR-509-3p, one of the miRNAs involved in the posttranscriptional regulation of the CFTR disease-gene of Cystic Fibrosis, and bearing suitable functionalization at its N- and C-ends aimed at improving its resistance to nucleases and cellular uptake, is able to revert the expression of the luciferase gene containing the 3′UTR of the gene in A549 human lung cancer cells, in agreement with the MD results that pointed at the formation of a stable RNA/PNA heteroduplex notwithstanding the short sequence of the latter. The here reported results widen the interest towards the use of small PNAs as effective anti-miRNA agents.
- Subjects :
- Peptide Nucleic Acids
Article Subject
Cystic Fibrosis
Ultraviolet Rays
lcsh:Medicine
Electrophoretic Mobility Shift Assay
Biology
Molecular Dynamics Simulation
Nucleic Acid Denaturation
General Biochemistry, Genetics and Molecular Biology
chemistry.chemical_compound
Cell Line, Tumor
microRNA
Humans
Gene
Regulation of gene expression
General Immunology and Microbiology
Peptide nucleic acid
Circular Dichroism
lcsh:R
RNA
General Medicine
MicroRNAs
chemistry
Biochemistry
Gene Expression Regulation
Nucleic acid
Spectrophotometry, Ultraviolet
Fluorescein-5-isothiocyanate
Heteroduplex
Research Article
Subjects
Details
- ISSN :
- 23146141
- Volume :
- 2014
- Database :
- OpenAIRE
- Journal :
- BioMed research international
- Accession number :
- edsair.doi.dedup.....e84e6fea2f96dbdff0d32757b33001e0