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Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas
- Source :
- PLoS ONE, Vol 16, Iss 7, p e0254711 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species’ GRF genes and their expression patterns is still lacking. In this study, we characterized the 10 JcGRF genes. A detailed investigation into the physic nut GRF gene family is performed, including analysis of the exon-intron structure, conserved domains, conserved motifs, phylogeny, chromosomal locations, potential small RNA targets and expression profiles under both normal growth and abiotic stress conditions. Phylogenetic analysis indicated that the 10 JcGRF genes were classified into five groups corresponding to group I, II, III, IV and V. The analysis of conserved domains showed that the motifs of JcGRF genes were highly conserved in Jatropha curcas. Expression analysis based on RNA-seq and qRT-PCR showed that almost all JcGRF genes had the highest expression in seeds, but very low expression was detected in the non-seed tissues tested, and four JcGRF genes responded to at least one abiotic stress at at least one treatment point. Our research will provide an important scientific basis for further research on the potential functions of JcGRF genes in Jatropha curcas growth and development, and response to abiotic stress, and will eventually provide candidate genes for the breeding of Jatropha curcas.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Candidate gene
Physiology
Gene Expression
Jatropha
Plant Science
Biochemistry
01 natural sciences
Genome
Plant Resistance to Abiotic Stress
Gene expression
Phylogeny
Data Management
Regulation of gene expression
Genetics
Multidisciplinary
Ecology
Plant Anatomy
Eukaryota
Phylogenetic Analysis
Plants
Droughts
Phylogenetics
Experimental Organism Systems
Plant Physiology
Seeds
Medicine
Jatropha curcas
Genome, Plant
Research Article
Computer and Information Sciences
Arabidopsis Thaliana
Science
Brassica
Biology
Research and Analysis Methods
03 medical and health sciences
Model Organisms
Protein Domains
Plant and Algal Models
Plant-Environment Interactions
Gene family
Gene Regulation
Evolutionary Systematics
Plant Defenses
Grasses
Gene
Taxonomy
Evolutionary Biology
Abiotic stress
Plant Ecology
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Proteins
Plant Pathology
biology.organism_classification
030104 developmental biology
Animal Studies
Rice
010606 plant biology & botany
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....e86150015b8b70eae6b2b209bc0547c5