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Uptake and Release of Neurotransmitters
- Source :
- Current Protocols in Neuroscience
- Publication Year :
- 1998
- Publisher :
- Wiley, 1998.
-
Abstract
- The availability of clonal cell lines for norepinephrine, dopamine, and serotonin transporters allows the characterization of drug interactions with transporter recognition sites using radioligands, as well as the characterization of drug effects on selective transporter-mediated uptake and release of substrate. In addition to clonal cell lines, synaptosomes prepared from specific brain regions can be used to conduct these studies without interference by endogenous transporters or binding proteins that are present in other tissues. This unit presents protocols for uptake and release of tritiated substrates using intact cells (either detached or in suspension) or synaptosomes. An HPLC procedure for electrochemical detection of nonradiolabeled substrates is also provided. Time-dependent release can also be measured in assays involving real-time sampling.
- Subjects :
- Dopamine
Recombinant Fusion Proteins
Endogeny
Biology
Kidney
Cell Line
Receptors, Dopamine
Radioligand Assay
Norepinephrine
Electrochemistry
medicine
Animals
Humans
Receptor
Chromatography, High Pressure Liquid
Dopamine Plasma Membrane Transport Proteins
Neurotransmitter Agents
Psychotropic Drugs
General Neuroscience
Transporter
Corpus Striatum
Clone Cells
Rats
Biochemistry
Cell culture
Serotonin
Synaptosomes
medicine.drug
Subjects
Details
- ISSN :
- 19348576 and 19348584
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Current Protocols in Neuroscience
- Accession number :
- edsair.doi.dedup.....e8651d97a5d1e21ab50ad1f26be2d6bf
- Full Text :
- https://doi.org/10.1002/0471142301.ns0709s02