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Uptake and Release of Neurotransmitters

Authors :
Kim A. Neve
Amy J. Eshleman
Aaron Janowsky
Source :
Current Protocols in Neuroscience
Publication Year :
1998
Publisher :
Wiley, 1998.

Abstract

The availability of clonal cell lines for norepinephrine, dopamine, and serotonin transporters allows the characterization of drug interactions with transporter recognition sites using radioligands, as well as the characterization of drug effects on selective transporter-mediated uptake and release of substrate. In addition to clonal cell lines, synaptosomes prepared from specific brain regions can be used to conduct these studies without interference by endogenous transporters or binding proteins that are present in other tissues. This unit presents protocols for uptake and release of tritiated substrates using intact cells (either detached or in suspension) or synaptosomes. An HPLC procedure for electrochemical detection of nonradiolabeled substrates is also provided. Time-dependent release can also be measured in assays involving real-time sampling.

Details

ISSN :
19348576 and 19348584
Volume :
2
Database :
OpenAIRE
Journal :
Current Protocols in Neuroscience
Accession number :
edsair.doi.dedup.....e8651d97a5d1e21ab50ad1f26be2d6bf
Full Text :
https://doi.org/10.1002/0471142301.ns0709s02