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Loss of cardiac magnesium in experimental heart failure prolongs and destabilizes repolarization in dogs
- Source :
- Journal of the American College of Cardiology. 31(3)
- Publication Year :
- 1998
-
Abstract
- Objectives. We sought to determine whether heart failure results in loss of cardiac magnesium sufficient to alter cellular electrophysiology. Background. Free magnesium has numerous intracellular roles affecting metabolism, excitability and RNA synthesis. Total cardiac magnesium content is reduced in heart failure, but it is unclear whether magnesium loss is primary or iatrogenic. Furthermore, it is unknown whether free magnesium levels are affected or whether a change in free magnesium would alter cellular electrophysiology. Methods. Eight mongrel dogs underwent demand ventricular pacing (VVI) at 250 beats/min for 3 weeks to induce heart failure. Sublingual epithelial magnesium was measured before pacing and at death. Left ventricular myocytes were isolated and loaded with Mag-Indo-1 to measure free magnesium ([Mg2+]i); myocytes from eight normal dogs served as controls. To test whether changes in [Mg2+]iin this range could alter cellular repolarization, current-clamped myocytes were dialyzed with 0.5 or 1.0 mmol/liter MgCl2. Results. Mean sublingual epithelial magnesium fell significantly in the paced animals, from 36.9 ± 0.5 to 33.9 ± 0.7 mEq/liter (p < 0.01). Mean cardiac [Mg2+]iwas significantly lower in the dogs with heart failure—0.49 ± 0.06 versus 1.06 ± 0.15 mmol/liter (p < 0.003). Time to 90% repolarization was significantly shorter in cells dialyzed with 1.0 mmol/liter compared with 0.5 mmol/liter MgCl2in myocytes from normal dogs or dogs with heart failure (596 ± 34 vs. 760 ± 58 ms in normal dogs and 586 ± 29 vs. 838 ± 98 ms in dogs with heart failure; p < 0.05 for each). Conclusions. Experimental heart failure results in both tissue and cardiac magnesium loss in the absence of drug therapy. Free cardiac magnesium is significantly reduced, possibly contributing to abnormal repolarization in heart failure.
- Subjects :
- medicine.medical_specialty
Heart disease
chemistry.chemical_element
Action Potentials
Dogs
Heart Conduction System
Internal medicine
Medicine
Myocyte
Repolarization
Animals
Magnesium
Magnesium ion
Diminution
Heart Failure
biology
business.industry
Myocardium
Fissipedia
biology.organism_classification
medicine.disease
Electrophysiology
Disease Models, Animal
Endocrinology
chemistry
Heart failure
business
Cardiology and Cardiovascular Medicine
Subjects
Details
- ISSN :
- 07351097
- Volume :
- 31
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of the American College of Cardiology
- Accession number :
- edsair.doi.dedup.....e878f66137e998677f2a940df0c6b403