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Role of host tissues for sustained humoral effects after endothelial progenitor cell transplantation into the ischemic heart
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 2007
- Publisher :
- Rockefeller University Press, 2007.
-
Abstract
- Noncellular differentiation effects have emerged as important mechanisms mediating therapeutic effects of stem or progenitor cell transplantation. Here, we investigated the expression patterns and sources of humoral factors and their regional and systemic biological effects after bone marrow (BM)-derived endothelial progenitor cell (EPC) transplantation into ischemic myocardium. Although most of the transplanted EPCs disappeared within a week, up-regulation of multiple humoral factors was sustained for longer than two weeks, which correlated well with the recovery of cardiac function. To determine the source of the humoral factors, we injected human EPCs into immunodeficient mice. Whereas the expression of human EPC (donor)-derived cytokines rapidly decreased to a nondetectable level within a week, up-regulation of mouse (recipient)-derived cytokines, including factors that could mobilize BM cells, was sustained. Histologically, we observed higher capillary density, a higher proliferation of myocardial cells, a lower cardiomyocyte apoptosis, and reduced infarct size. Furthermore, after EPC transplantation, BM-derived stem or progenitor cells were increased in the peripheral circulation and incorporated into the site of neovascularization and myocardial repair. These data indicate that myocardial EPC transplantation induces humoral effects, which are sustained by host tissues and play a crucial role in repairing myocardial injury.
- Subjects :
- Cell Transplantation
medicine.medical_treatment
Immunology
Myocardial Infarction
Myocardial Ischemia
Ischemia
Apoptosis
Mice, SCID
030204 cardiovascular system & hematology
Biology
Models, Biological
Endothelial progenitor cell
Article
Neovascularization
Mice
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Humans
Immunology and Allergy
Progenitor cell
Cell Proliferation
030304 developmental biology
0303 health sciences
Stem Cells
Endothelial Cells
Heart
Articles
medicine.disease
Mice, Inbred C57BL
Transplantation
medicine.anatomical_structure
Cytokine
Antibody Formation
cardiovascular system
Bone marrow
Stem cell
medicine.symptom
Subjects
Details
- ISSN :
- 15409538 and 00221007
- Volume :
- 204
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....e882fbb75fe33519b139390fabc5549b