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Long Noncoding RNA CASC2 Facilitated Wound Healing through miRNA-155/HIF-1α in Diabetic Foot Ulcers

Authors :
Minjie He
Liang Tu
Ruo Shu
Qi Meng
Sicheng Du
Zhao Xu
Shaoyun Wang
Source :
Contrast Media & Molecular Imaging. 2022:1-12
Publication Year :
2022
Publisher :
Hindawi Limited, 2022.

Abstract

Diabetic foot ulcers (DFU) are among the serious complications which are closely linked to diabetes mellitus. However, there is still a lack of accurate and effective standard prevention and treatment programs for DFU. In this manuscript, we have investigated the function of lncRNA cancer susceptibility candidate 2 (CASC2)/miR-155/hypoxia-inducible factor 1-alpha (HIF-1α) in the wound healing of DFU. We have analyzed lncRNA CASC2`s expression in the marginal tissues of ulcers in patients and mice with DFU. Additionally, the interaction relationship and mechanism between lncRNA CASC2, miR-155, and HIF-1α were determined, which proved the effects of lncRNA CASC2/miR-155/HIF-1α on fibroblasts apoptosis, proliferation, and migration. According to our study, the lncRNA CASC2’s expression was low in the tissues of ulcers of DFU mice and patients. lncRNA CASC2’s overexpression promoted fibroblasts migration, proliferation, and inhibited apoptosis and was beneficial for the healing of wounds, preferably in the DFU mice. In addition, lncRNA CASC2 directly targets miR-155 and HIF-1α functions as miR-155’s target gene. Overexpression of miR-155 abrogated the function of lncRNA CASC2. Similarly, HIF-1α’s inhibition has reversed the effect of miR-155 downregulation on fibroblasts. In general, overexpression of lncRNA CASC2 facilitated wound healing through miR-155/HIF-1α in DFU.

Details

ISSN :
15554317 and 15554309
Volume :
2022
Database :
OpenAIRE
Journal :
Contrast Media & Molecular Imaging
Accession number :
edsair.doi.dedup.....e8a898eb5ca89eb69f2ef95196e04f1c
Full Text :
https://doi.org/10.1155/2022/6291497