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Multiple Roles for Slits in the Control of Cell Migration in the Rostral Migratory Stream
- Source :
- Journal of Neuroscience, Journal of Neuroscience, Society for Neuroscience, 2004, 24, pp.1497-1506. ⟨10.1523/JNEUROSCI.4729-03.2004⟩, Journal of Neuroscience, 2004, 24, pp.1497-1506. ⟨10.1523/JNEUROSCI.4729-03.2004⟩
- Publication Year :
- 2004
- Publisher :
- Society for Neuroscience, 2004.
-
Abstract
- The subventricular zone (SVZ) contains undifferentiated cells, which proliferate and generate olfactory bulb (OB) interneurons. Throughout life, these cells leave the SVZ and migrate along the rostral migratory stream (RMS) to the OB where they differentiate.In vitro, the septum and the choroid plexus (CP) secrete repulsive factors that could orient the migration of OB precursors. Slit1 and Slit2, two known chemorepellents for developing axons, can mimic this effect. We show here that the Slit receptors Robo2 and Robo3/Rig-1 are expressed in the SVZ and the RMS and that Slit1 and Slit2 are still present in the adult septum. UsingSlit1/2-deficient mice, we found that Slit1 and Slit2 are responsible for both the septum and the CP repulsive activityin vitro. In adult mice lacking Slit1, small chains of SVZ-derived cells migrate caudally into the corpus callosum, supporting a role for Slits in orienting the migration of SVZ cells. Surprisingly, in adult mice, Slit1 was also expressed by type A and type C cells in the SVZ and RMS, suggesting that Slit1 could act cell autonomously. This hypothesis was tested using cultures of SVZ explants or isolated neurospheres fromSlit1-/- orSlit1+/- mice. In both types of cultures, the migration of SVZ cells was altered in the absence of Slit1. This suggests that the regulation of the migration of OB precursors by Slit proteins is complex and not limited to repulsion.
- Subjects :
- Rostral migratory stream
animal diseases
Development/Plasticity/Repair
Subventricular zone
Mice, Transgenic
Nerve Tissue Proteins
Biology
Mice
03 medical and health sciences
0302 clinical medicine
Cell Movement
Genes, Reporter
Interneurons
Lateral Ventricles
Neurosphere
SLIT1
medicine
SLIT2
Animals
Receptors, Immunologic
Cells, Cultured
030304 developmental biology
Mice, Knockout
0303 health sciences
Chemotaxis
Stem Cells
General Neuroscience
Cell Differentiation
Cell migration
Olfactory Bulb
Slit
Olfactory bulb
Cell biology
medicine.anatomical_structure
nervous system
Choroid Plexus
Intercellular Signaling Peptides and Proteins
Septum of Brain
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Neuroscience
Cell Division
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....e8afd5ba7e904aabbade1e4039f7acac