Back to Search
Start Over
Ninety-Day Nephrotoxicity Evaluation of 3-MCPD 1-Monooleate and 1-Monostearate Exposures in Male Sprague Dawley Rats Using Proteomic Analysis
- Source :
- Journal of Agricultural and Food Chemistry. 68:2765-2772
- Publication Year :
- 2020
- Publisher :
- American Chemical Society (ACS), 2020.
-
Abstract
- Fatty acid esters of 3-monochloropropane 1,2-diol (3-MCPD esters) are processing-induced food toxicants, with the kidney as their major target organ. For the first time, this study treated Sprague Dawley (SD) rats with 3-MCPD 1-monooleate at 10 and 100 mg/kg BW/day and 1-monostearate at 15 and 150 mg/kg BW/day for 90 days and examined for their potential semi-long-term nephrotoxicity and the associated molecular mechanisms. No bodyweight difference was observed between groups during the study. Both 3-MCPD 1-monooleate and 1-monostearate resulted in a dose-dependent increase of serum urea creatinine, uric acid and urea nitrogen levels, and histological renal impairment. The proteomic analysis of the kidney samples showed that the 3-MCPD esters deregulated proteins involved in the pathways for ion transportation, apoptosis, the metabolism of xenobiotics, and enzymes related to endogenous biological metabolisms of carbohydrates, amino acids, nitrogen, lipids, fatty acids, and the tricarboxylic acid (TCA) cycle, providing partial explanation for the nephrotoxicity of 3-MCPD esters.
- Subjects :
- Male
Proteomics
0106 biological sciences
medicine.medical_specialty
alpha-Chlorohydrin
Kidney
01 natural sciences
Nephrotoxicity
Rats, Sprague-Dawley
chemistry.chemical_compound
Internal medicine
Stearates
medicine
Animals
Humans
chemistry.chemical_classification
Creatinine
010401 analytical chemistry
Fatty acid
Esters
General Chemistry
Tricarboxylic acid
Metabolism
Rats
Uric Acid
0104 chemical sciences
Amino acid
medicine.anatomical_structure
Endocrinology
chemistry
Uric acid
Kidney Diseases
General Agricultural and Biological Sciences
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....e92200db4ddb8a0980bf0a2723a321e6