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Facing Two T Cell Epitopes: A Degree of Randomness in the Primary Response Is Lost Upon Secondary Immunization
- Source :
- ResearcherID
- Publication Year :
- 2000
- Publisher :
- The American Association of Immunologists, 2000.
-
Abstract
- We have analyzed the hierarchy of epitope-specific T cell populations during a primary and a secondary CD8 T cell response. MHC-peptide tetramers were used to track the in vivo kinetics of expansion of T cell populations specific for two Kd-restricted epitopes simultaneously presented by a murine tumor cell following primary or recall immunizations. Individual syngeneic mice generated remarkably different primary CTL responses, as reflected by up to 60-fold differences in the relative contribution of each peptide-specific T cell population to the overall response. In these primary immunizations, the CTL dominance was not dictated by the respective abundance of the presented epitopes. In sharp contrast, the secondary response was systematically associated with a selective expansion of the same epitope-specific population both in vitro and in vivo. In vitro experiments indicated that the extent of expansion of each epitope-specific memory population is modulated by the epitope density. We conclude that, at least for this set of epitopes, the CTL hierarchy is not controlled by the same parameters in a primary vs a secondary response.
- Subjects :
- T cell
Immunology
Cell
Population
Immunization, Secondary
Epitopes, T-Lymphocyte
Mast-Cell Sarcoma
Biology
Lymphocyte Activation
Epitope
Evolution, Molecular
Mice
T-Lymphocyte Subsets
In vivo
Tumor Cells, Cultured
medicine
Animals
Immunology and Allergy
Cytotoxic T cell
education
Antigen Presentation
education.field_of_study
H-2 Antigens
Adoptive Transfer
In vitro
Clone Cells
CTL
medicine.anatomical_structure
Mice, Inbred DBA
Methionine Sulfoxide Reductases
Female
Oxidoreductases
Immunologic Memory
Oligopeptides
T-Lymphocytes, Cytotoxic
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 165
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....e93e4a10505be0005e7d5861a3f78305