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MAP Kinase driven actomyosin rearrangement is a crucial regulator of monocyte to macrophage differentiation
- Source :
- Cellular Signalling. 73:109691
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Rearrangement of actin cytoskeleton correlates significantly with the immune responses as the perturbation of cytoskeletal dynamics leads to many immune deficiencies. Mechanistic insights into this correlation remain unknown. Cellular spreading, the most characteristic phenotype associated with monocyte to macrophage differentiation, led us to investigate the contribution of actomyosin dynamics in monocyte differentiation. Our observation revealed that actomyosin reorganization intrinsically governs the process of monocyte to macrophage differentiation. Further, we established that the MAPK-driven signaling pathways regulate the cellular actomyosin dynamics that direct monocyte to macrophage differentiation. We also identified P42/44 Mitogen-Activated Protein Kinase (P42/44 MAPK), P38 Mitogen-Activated Protein Kinase (P38 MAPK), MAP Kinase Activated Protein Kinase 2 (MK-2), Heat Shock Protein 27 (Hsp-27), Lim Kinase (Lim K), non-muscle cofilin (n-cofilin), Myosin Light Chain Kinase (MLCK) and Myosin Light Chain (MLC) as critical components of the signaling network. Moreover, we have shown the involvement of the same signaling cascade in 3D gel-like microenvironment induced spontaneous monocyte to macrophage differentiation and in human blood-derived PBMC differentiation. Our study reveals new mechanistic insights into the process of monocyte to macrophage differentiation.
- Subjects :
- 0301 basic medicine
Myosin light-chain kinase
THP-1 Cells
macromolecular substances
Monocytes
Lim kinase
03 medical and health sciences
0302 clinical medicine
medicine
Humans
Protein kinase A
biology
Chemistry
Macrophages
Monocyte
Cell Differentiation
Actomyosin
Cell Biology
Actin cytoskeleton
Cell biology
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Monocyte differentiation
Mitogen-activated protein kinase
biology.protein
Mitogen-Activated Protein Kinases
Signal transduction
Signal Transduction
Subjects
Details
- ISSN :
- 08986568
- Volume :
- 73
- Database :
- OpenAIRE
- Journal :
- Cellular Signalling
- Accession number :
- edsair.doi.dedup.....e975c3b9ca0cdbd2604fea71e75f351a