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Metre-long cell-laden microfibres exhibit tissue morphologies and functions
Metre-long cell-laden microfibres exhibit tissue morphologies and functions
- Source :
- Nature Materials. 12:584-590
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Artificial reconstruction of fibre-shaped cellular constructs could greatly contribute to tissue assembly in vitro. Here we show that, by using a microfluidic device with double-coaxial laminar flow, metre-long core-shell hydrogel microfibres encapsulating ECM proteins and differentiated cells or somatic stem cells can be fabricated, and that the microfibres reconstitute intrinsic morphologies and functions of living tissues. We also show that these functional fibres can be assembled, by weaving and reeling, into macroscopic cellular structures with various spatial patterns. Moreover, fibres encapsulating primary pancreatic islet cells and transplanted through a microcatheter into the subrenal capsular space of diabetic mice normalized blood glucose concentrations for about two weeks. These microfibres may find use as templates for the reconstruction of fibre-shaped functional tissues that mimic muscle fibres, blood vessels or nerve networks in vivo.
- Subjects :
- Male
Materials science
Alginates
Cellular differentiation
Cell
Islets of Langerhans Transplantation
Biocompatible Materials
Nanotechnology
Hydrogel, Polyethylene Glycol Dimethacrylate
Diabetes Mellitus, Experimental
Extracellular matrix
Islets of Langerhans
Mice
In vivo
Human Umbilical Vein Endothelial Cells
medicine
Animals
Humans
Myocyte
Myocytes, Cardiac
General Materials Science
Mice, Inbred BALB C
Muscle Cells
Tissue Engineering
Mechanical Engineering
Cell Differentiation
Diabetic mouse
General Chemistry
Microfluidic Analytical Techniques
Condensed Matter Physics
In vitro
Extracellular Matrix
Rats
medicine.anatomical_structure
Mechanics of Materials
NIH 3T3 Cells
Biophysics
Adult stem cell
Subjects
Details
- ISSN :
- 14764660 and 14761122
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Nature Materials
- Accession number :
- edsair.doi.dedup.....e99789a3c924128faef440773429c624
- Full Text :
- https://doi.org/10.1038/nmat3606