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Effects of mixed meal tolerance test on gastric emptying, glucose and lipid homeostasis in obese nonhuman primates
- Source :
- Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021), Scientific Reports
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Meal ingestion elicits a variety of neuronal, physiological and hormonal responses that differ in healthy, obese or diabetic individuals. The mixed meal tolerance test (MMTT) is a well-established method to evaluate pancreatic β-cell reserve and glucose homeostasis in both preclinical and clinical research in response to calorically defined meal. Nonhuman primates (NHPs) are highly valuable for diabetic research as they can naturally develop type 2 diabetes mellitus (T2DM) in a way similar to the onset and progression of human T2DM. The purpose of this study was to investigate the reproducibility and effects of a MMTT containing acetaminophen on plasma glucose, insulin, C-peptide, incretin hormones, lipids, acetaminophen appearance (a surrogate marker for gastric emptying) in 16 conscious obese cynomolgus monkeys (Macaca fascicularis). Plasma insulin, C-peptide, TG, aGLP-1, tGIP, PYY and acetaminophen significantly increased after meal/acetaminophen administration. A subsequent study in 6 animals showed that the changes of plasma glucose, insulin, C-peptide, lipids and acetaminophen were reproducible. There were no significant differences in responses to the MMTT among the obese NHPs with (n = 11) or without (n = 5) hyperglycemia. Our results demonstrate that mixed meal administration induces significant secretion of several incretins which are critical for maintaining glucose homeostasis. In addition, the responses to the MMTTs are reproducible in NHPs, which is important when the MMTT is used for evaluating post-meal glucose homeostasis in research.
- Subjects :
- Blood Glucose
Male
0301 basic medicine
medicine.medical_specialty
medicine.medical_treatment
Science
Incretin
030209 endocrinology & metabolism
Gastric Inhibitory Polypeptide
Incretins
Article
Gastrointestinal Hormones
03 medical and health sciences
0302 clinical medicine
Insulin-Secreting Cells
Internal medicine
medicine
Animals
Homeostasis
Insulin
Glucose homeostasis
Acetaminophen
Meal
Multidisciplinary
C-Peptide
Gastric emptying
business.industry
digestive, oral, and skin physiology
Reproducibility of Results
Type 2 Diabetes Mellitus
Glucose Tolerance Test
Animal Feed
Lipids
Metabolic syndrome
Macaca fascicularis
Glucose
Metabolism
030104 developmental biology
Endocrinology
Diabetes Mellitus, Type 2
Gastric Emptying
Medicine
business
Hormone
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....e9fee7208f4019cd0a8235b25bd02e3a