SAT-568 Glutamine Metabolism Is a New Potential Therapeutic Target in Aggressive Thyroid Cancer

Thyroid cancer is the most frequent endocrine tumor. BRAF mutation is the most frequent alteration in thyroid cancer. The metabolic consequences of these mutations is unknown. Recent studies have suggested a pro-metastatic role of the metabolic processes in several solid malignancies. The aim of this study is to identify the metabolic pathways dysregulated in thyroid carcinogenesis with oncogenic mutations. BRAFV600Einduced HEK293 cells underwent a metabolomic profiling via mass spectrometry, which showed an increase in glutamine metabolism. Glutaminolysis, a dysregulated metabolic pathway is initiated by glutaminase (GLS), which converts glutamine to glutamate. Analysis of GLS expression in thyroid cancer tissue revealed an increase of GLS within the tumor compared to normal tissue. GEO dataset analysis revealed higher GLS expression in undifferentiated thyroid tumors compared to differentiated cancer and normal tissue (p=0.0001, p