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Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins
- Source :
- The Journal of biological chemistry. 285(21)
- Publication Year :
- 2010
-
Abstract
- In eukaryotes, DNA replication is fired once in a single cell cycle before cell division starts to maintain stability of the genome. This event is tightly controlled by a series of proteins. Cdt1 is one of the licensing factors and is involved in recruiting replicative DNA helicase Mcm2-7 proteins into the pre-replicative complex together with Cdc6. In Cdt1, the C-terminal region serves as a binding site for Mcm2-7 proteins, although the details of these interactions remain largely unknown. Here, we report the structure of the region and the key residues for binding to Mcm proteins. We determined the solution structure of the C-terminal fragment, residues 450-557, of mouse Cdt1 by NMR. The structure consists of a winged-helix domain and shows unexpected similarity to those of the C-terminal domain of Cdc6 and the central fragment of Cdt1, thereby implying functional and evolutionary relationships. Structure-based mutagenesis and an in vitro binding assay enabled us to pinpoint the region that interacts with Mcm proteins. Moreover, by performing in vitro binding and budding yeast viability experiments, we showed that approximately 45 residues located in the N-terminal direction of the structural region are equally crucial for recognizing Mcm proteins. Our data suggest the possibility that winged-helix domain plays a role as a common module to interact with replicative helicase in the DNA replication-licensing process.
- Subjects :
- Cell Cycle Proteins
MADS Domain Proteins
DNA and Chromosomes
Biochemistry
Protein Structure, Secondary
DNA replication factor CDT1
Mice
Structure-Activity Relationship
Protein structure
Animals
Molecular Biology
Nuclear Magnetic Resonance, Biomolecular
Telomere-binding protein
Genetics
biology
DNA replication
DNA Helicases
Helicase
Nuclear Proteins
Cell Biology
Cell biology
Protein Structure, Tertiary
DNA binding site
DNA-Binding Proteins
Licensing factor
Mutagenesis
biology.protein
Binding domain
Protein Binding
Subjects
Details
- ISSN :
- 1083351X
- Volume :
- 285
- Issue :
- 21
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....ea3844c12bf7331f4c5fa7b58886b974