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Synergistic Effects of Kartogenin and Transforming Growth Factor‐β3 on Chondrogenesis of Human Umbilical Cord Mesenchymal Stem Cells In Vitro
- Source :
- Orthopaedic Surgery, Orthopaedic Surgery, Vol 12, Iss 3, Pp 938-945 (2020)
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Objective To explore the effect of kartogenin (KGN) on proliferation and chondrogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSC) in vitro, and the synergistic effects of KGN and transforming growth factor (TGF)‐β3 on hUCMSC. Methods Human umbilical cord mesenchymal stem cells were isolated and cultured. Then the differentiation properties were identified by flow cytometry analysis. HUCMSC were divided into four groups: control group, KGN group, TGF‐β3 group, and TK group (with TGF‐β3 and KGN added into the medium simultaneously). Cells in all groups were induced for 21 days using the suspension ball culture method. Hematoxylin and eosin, immunofluorescence, and Alcian blue staining were used to analyze chondrogenic differentiation. Real‐time reverse transcriptase polymerase chain reaction was performed to investigate genes associated with chondrogenic differentiation. Result Hematoxylin and eosin staining showed that cells in the TGF‐β3 group and the TK group had formed cartilage‐like tissue after 21 days of culture. The results of immunofluorescence and Alcian blue staining showed that compared with the control group, cells in the KGN and TGF‐β3 groups demonstrated increased secretion of aggrecan after 21 days of culture. In addition, cells in the group combining KGN with TGF‐β3 (5.587 ± 0.27, P
- Subjects :
- Scientific Articles
Phthalic Acids
H&E stain
Umbilical cord
Umbilical Cord
Flow cytometry
Andrology
03 medical and health sciences
Transforming Growth Factor beta3
0302 clinical medicine
lcsh:Orthopedic surgery
medicine
Humans
Anilides
Scientific Article
Orthopedics and Sports Medicine
Transforming growth factor‐β3
Cells, Cultured
Aggrecan
030222 orthopedics
medicine.diagnostic_test
Chemistry
Mesenchymal stem cell
Kartogenin
Chondrogenesis
In vitro
lcsh:RD701-811
medicine.anatomical_structure
Mesenchymal stem cells
Surgery
030217 neurology & neurosurgery
Transforming growth factor
Subjects
Details
- ISSN :
- 17577861 and 17577853
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Orthopaedic Surgery
- Accession number :
- edsair.doi.dedup.....ea44d90c3e3ee711ea61091df8e1c643