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Primate foamy virus Pol proteins are imported into the nucleus
- Source :
- Journal of General Virology. 81:2941-2947
- Publication Year :
- 2000
- Publisher :
- Microbiology Society, 2000.
-
Abstract
- Mouse monoclonal antibodies (MAbs) that specifically detect the 127 kDa Pol precursor and the 85 kDa reverse transcriptase/RNase H (RT/RN) or pr127 and the 40 kDa integrase (IN) in immunoblot and immunofluorescence assays (IFA) were used to investigate the subcellular localization of primate foamy virus (PFV) proteins. IFA of cells infected with PFV using the anti-Pol MAbs and rabbit anti-capsid (Gag) serum revealed that both the Gag and Pol proteins are transported into the nucleus. Transfection of cells with eukaryotic expression constructs for pr127Pol, p85RT/RN and p40IN served to show Gag-independent subcellular localization of Pol proteins. Interestingly, not only the Pol precursor and IN molecules were found to be localized to the nucleus, but also the RT/RN subdomain. It is therefore suggested that PFV cores bear at least three separate nuclear localization signals, one in Gag and two in Pol. The latter appear to be localized to the two Pol subdomains.
- Subjects :
- Primates
viruses
Blotting, Western
Nuclear Localization Signals
Ribonuclease H
Active Transport, Cell Nucleus
Fluorescent Antibody Technique
Gene Products, gag
Gene Products, pol
Antibodies, Viral
Transfection
Immunofluorescence
Virus
Cell Line
Mice
Cricetinae
Virology
medicine
Animals
RNase H
Cell Nucleus
Integrases
medicine.diagnostic_test
biology
Antibodies, Monoclonal
RNA-Directed DNA Polymerase
3T3 Cells
Subcellular localization
Molecular biology
Reverse transcriptase
Integrase
Mice, Inbred C57BL
biology.protein
Spumavirus
Nuclear localization sequence
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Volume :
- 81
- Database :
- OpenAIRE
- Journal :
- Journal of General Virology
- Accession number :
- edsair.doi.dedup.....ea511e7e7cd1eeeb24c800b7b7fcd5f1