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Real-time PCR for detection of Brucella spp. DNA in human serum samples

Authors :
Pierre-Alain Falconnet
M Maurin
C Debeaumont
Laboratoire de Bactériologie
CHU Grenoble
Laboratoire Adaptation et pathogénie des micro-organismes [Grenoble] (LAPM)
Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)
Source :
European Journal of Clinical Microbiology and Infectious Diseases, European Journal of Clinical Microbiology and Infectious Diseases, Springer Verlag, 2005, 24 (12), pp.842-5. ⟨10.1007/s10096-005-0064-0⟩
Publication Year :
2005
Publisher :
Springer Science and Business Media LLC, 2005.

Abstract

Presented here are the results of an evaluation of an in-house real-time PCR assay for the rapid and specific diagnosis of human brucellosis. The assay was based on direct amplification from serum samples of a 169-bp portion of bcsp31, a gene found in all Brucella species and biovars. Species specificity and selectivity of this real-time PCR assay were evaluated using genomic DNA from 15 Brucella strains and 42 non-Brucella strains, and the results were 100%. Among 17 culture-proven brucellosis patients, sera from 11 gave a positive amplification signal, corresponding to a sensitivity of 64.7%. In contrast, negative results were obtained for all sera from 60 control patients, corresponding to a specificity of 100%. The results indicate this test is well adapted for definite confirmation of brucellosis cases, when Brucella cultures remain sterile and serological tests demonstrate the presence of cross-reacting antibodies against Brucella sp. and Yersinia enterocolitica O:9 antigens.

Details

ISSN :
14354373 and 09349723
Volume :
24
Database :
OpenAIRE
Journal :
European Journal of Clinical Microbiology & Infectious Diseases
Accession number :
edsair.doi.dedup.....ea7c1abb62f86829e70b9b42aff15f05