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Real-time PCR for detection of Brucella spp. DNA in human serum samples
- Source :
- European Journal of Clinical Microbiology and Infectious Diseases, European Journal of Clinical Microbiology and Infectious Diseases, Springer Verlag, 2005, 24 (12), pp.842-5. ⟨10.1007/s10096-005-0064-0⟩
- Publication Year :
- 2005
- Publisher :
- Springer Science and Business Media LLC, 2005.
-
Abstract
- Presented here are the results of an evaluation of an in-house real-time PCR assay for the rapid and specific diagnosis of human brucellosis. The assay was based on direct amplification from serum samples of a 169-bp portion of bcsp31, a gene found in all Brucella species and biovars. Species specificity and selectivity of this real-time PCR assay were evaluated using genomic DNA from 15 Brucella strains and 42 non-Brucella strains, and the results were 100%. Among 17 culture-proven brucellosis patients, sera from 11 gave a positive amplification signal, corresponding to a sensitivity of 64.7%. In contrast, negative results were obtained for all sera from 60 control patients, corresponding to a specificity of 100%. The results indicate this test is well adapted for definite confirmation of brucellosis cases, when Brucella cultures remain sterile and serological tests demonstrate the presence of cross-reacting antibodies against Brucella sp. and Yersinia enterocolitica O:9 antigens.
- Subjects :
- Male
Bacteremia
Brucellaceae
Polymerase Chain Reaction
law.invention
Serology
law
MESH: Child
Child
MESH: Bacteremia
Yersinia enterocolitica
MESH: Bacterial Proteins
Polymerase chain reaction
MESH: Aged
0303 health sciences
MESH: Middle Aged
biology
MESH: Brucella
General Medicine
Middle Aged
Infectious Diseases
Real-time polymerase chain reaction
Female
MESH: Membrane Proteins
Adult
DNA, Bacterial
MESH: DNA Primers
Microbiology (medical)
Brucella
Brucellosis
Microbiology
03 medical and health sciences
Bacterial Proteins
MESH: Brucellosis
medicine
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Serotyping
Aged
DNA Primers
030304 developmental biology
MESH: Humans
030306 microbiology
Membrane Proteins
MESH: Serotyping
MESH: Adult
MESH: Polymerase Chain Reaction
biology.organism_classification
medicine.disease
MESH: DNA, Bacterial
Virology
MESH: Male
genomic DNA
MESH: Female
Subjects
Details
- ISSN :
- 14354373 and 09349723
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- European Journal of Clinical Microbiology & Infectious Diseases
- Accession number :
- edsair.doi.dedup.....ea7c1abb62f86829e70b9b42aff15f05