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Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures
- Source :
- Biochemistry. 23(16)
- Publication Year :
- 1984
-
Abstract
- Two hybridomas producing monoclonal antibodies to poly(adenosine diphosphate ribose) [poly(ADP-Rib)] were established. One antibody, 10H (IgG3, kappa), bound to most of the poly(ADP-Rib) preparation, which consisted of molecules of various sizes of more than 20 ADP-Rib residues. The binding of this antibody was inhibited by not only poly-(ADP-Rib) but also a monomer unit of poly(ADP-Rib), Ado(P)-Rib-P. The sites protected by antibody 10H were isolated and analyzed by hydrolysis with alkaline phosphomonoesterase and then snake venom phosphodiesterase. The sites contained the same amounts of monomer units and branched portions [Ado(P)-Rib(P)-Rib-P] as the original poly(ADP-Rib) molecules but a lower average number of branched portions per molecule than in the original molecules. The other antibody, 16B (IgM, lambda), reacted with only 50% of the radioactive poly(ADP-Rib), and its binding was not inhibited by a monomer unit. This antibody protected 25% of all the poly(ADP-Rib) molecules from hydrolysis by snake venom phosphodiesterase. The protected sites contained twice as many branched portions per molecule as the original poly(ADP-Rib) molecules. These results show that the two monoclonal antibodies recognize different structures of poly-(ADP-Rib); 10H antibody recognizes the linear structure with ribose-ribose linkages, and 16B antibody may recognize specific structures, including the branched portions of poly-(ADP-Rib).
- Subjects :
- musculoskeletal diseases
Poly Adenosine Diphosphate Ribose
medicine.drug_class
Antigen-Antibody Complex
Monoclonal antibody
Biochemistry
chemistry.chemical_compound
Hydrolysis
Epitopes
Mice
Structure-Activity Relationship
medicine
Molecule
Animals
Alkaline phosphomonoesterase
Mice, Inbred BALB C
Hybridomas
biology
Adenosine diphosphate ribose
Nucleoside Diphosphate Sugars
Antibodies, Monoclonal
musculoskeletal system
Kinetics
Monomer
chemistry
biology.protein
Female
Antibody
Subjects
Details
- ISSN :
- 00062960
- Volume :
- 23
- Issue :
- 16
- Database :
- OpenAIRE
- Journal :
- Biochemistry
- Accession number :
- edsair.doi.dedup.....ea8268d272cb66642c1cb9c3b3cc9c8a