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Antinociceptive and anti-arthritic effects of kramecyne
- Source :
- Life Sciences. 121:70-77
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Aims The aim of this study was to evaluate the antinociceptive (acute assays) and anti-inflammatory (chronic assays) effects of kramecyne (KACY), a peroxide isolated from Krameria cytisoides. Main methods The antinociceptive activity of KACY was evaluated using the hot plate, acetic acid and formalin tests. The effects of KACY on heat-induced hemolysis in rat erythrocytes were also evaluated. The in vivo anti-inflammatory assays were performed using the chronic TPA (12-O-tetradecanoylphorbol 13-acetate) method to induce ear edema and carrageenan-kaolin induced arthritis (CKIA). In the CKIA model, the hot plate test was performed, serum samples were obtained for the quantitation of pro-inflammatory (IL-1β, IL-6, IL-12 and TNF-α) and anti-inflammatory (IL-4 and IL-10) cytokines. Key findings KACY possess antinociceptive effects with comparable activity to naproxen (NPX). KACY inhibited hemolysis (EC50 = 180 μg/mL), in comparison to the untreated group and with a higher potency than NPX (EC50 = 263 μg/mL). KACY at 50 mg/kg decreased inflammation by 38% (chronic TPA-induced edema model) and by 26% (CKIA model), in comparison with the vehicle group and with similar activity to the positive controls 8 mg/kg indomethacin (IND) and 1 mg/kg methotrexate (MTX), respectively. In the CKIA model, KACY increased the release of anti-inflammatory (IL-4 and IL-10) cytokines but reduced the production of pro-inflammatory cytokines (IL-1β, IL-6, IL-12 and TNF-α). KACY at 50 and 100 mg/kg showed antinociceptive effects by 27% and 23%, respectively, in mice with mono-arthritis. Significance KACY might be a good alternative for the treatment of rheumatoid arthritis (RA) due its antinociceptive and anti-inflammatory activities.
- Subjects :
- Male
Naproxen
Arthritis
In Vitro Techniques
Pharmacology
Hemolysis
General Biochemistry, Genetics and Molecular Biology
Mice
chemistry.chemical_compound
Ethers, Cyclic
In vivo
Edema
medicine
Animals
Potency
Rats, Wistar
General Pharmacology, Toxicology and Pharmaceutics
Hot plate test
Pain Measurement
Analgesics
Chemistry
Anti-Inflammatory Agents, Non-Steroidal
Body Weight
Erythrocyte Membrane
General Medicine
medicine.disease
Peroxides
Rats
Carrageenan
Cytokines
Krameriaceae
medicine.symptom
medicine.drug
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 121
- Database :
- OpenAIRE
- Journal :
- Life Sciences
- Accession number :
- edsair.doi.dedup.....ea9973253c330b0e0bb75c91c36c55bf