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Correlation of clinical features and methylation status of MGMT gene promoter in glioblastomas
- Source :
- Journal of neuro-oncology. 68(3)
- Publication Year :
- 2004
-
Abstract
- In an effort to extend the potential relationship between the methylation status of MGMT promoter and response to CENU therapy, we examined the methylation status of MGMT promoter in 44 patients with glioblastomas. Tumor specimens were obtained during surgery before adjuvant treatment, frozen and stored at -80 degrees C until for DNA extraction process. DNA methylation patterns in the CpG island of the MGMT gene were determined in every tumor by methylation specific PCR (MSP). These results were then related to overall survival and response to alkylating agents using statistical analysis. Methylation of the MGMT promoter was detected in 68% of tumors, and 96.7% of methylated tumors exhibited also an unmethylated status. There was no relationship between the methylation status of the MGMT promoter and overall survival and response to alkylating agents. Our observations do not lead us to consider promoter methylation of MGMT gene as a prognostic factor of responsiveness to alkylating agents in glioblastomas.
- Subjects :
- Cancer Research
medicine.medical_treatment
Bisulfite sequencing
Biology
O(6)-Methylguanine-DNA Methyltransferase
medicine
Humans
Promoter Regions, Genetic
neoplasms
Gene
Antineoplastic Agents, Alkylating
Aged
Brain Neoplasms
Promoter
Methylation
DNA, Neoplasm
DNA Methylation
Middle Aged
Prognosis
DNA extraction
Combined Modality Therapy
digestive system diseases
Gene Expression Regulation, Neoplastic
Treatment Outcome
Neurology
Oncology
CpG site
DNA methylation
Immunology
Cancer research
Neurology (clinical)
Glioblastoma
Adjuvant
Nitroso Compounds
Subjects
Details
- ISSN :
- 0167594X
- Volume :
- 68
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of neuro-oncology
- Accession number :
- edsair.doi.dedup.....eaa69d17d510c8ce73c2d8454d66a925