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Novel three‐dimensional bioglass functionalized gelatin nanofibrous scaffolds for bone regeneration
- Source :
- Journal of Biomedical Materials Research Part B: Applied Biomaterials. 109:517-526
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- The clinical use of FDA-approved bone morphogenetic proteins (BMPs) are impeded by high costs, super-high dosage requirement, short half-life, and other undesirable side effects. Therefore, designing a biomaterial that can promote new bone formation without using exogenous BMPs is highly desirable in clinical applications. In the present work, a new kind of nanofibrous scaffold composed of gelatin and 45S5 bioglass (GF/45S5 BG) was prepared through thermally induced phase separation method together with the particle leach technique (TIPS&P). In addition to the significantly higher mechanical strength, the composite scaffolds (GF/45S5 BG) significantly increased osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro compared with the neat scaffold (GF) without adding other biological agents, for example, BMPs or hormones. Most importantly, our in vivo studies also indicated that GF/45S5 BG scaffolds could directly promote ectopic bone regeneration in SD rats without exogenous BMP2. In summary, both in vitro and in vivo results indicated that the novel 45S5 bioglass functionalized GF nanofibrous scaffold is a promising alternative for bone tissue engineering.
- Subjects :
- Male
Ceramics
Scaffold
Bone Regeneration
Materials science
food.ingredient
Nanofibers
Biomedical Engineering
02 engineering and technology
Bone morphogenetic protein
Bone morphogenetic protein 2
Gelatin
Rats, Sprague-Dawley
Biomaterials
03 medical and health sciences
food
Osteogenesis
In vivo
Materials Testing
Spectroscopy, Fourier Transform Infrared
Animals
Humans
Bone regeneration
030304 developmental biology
0303 health sciences
Tissue Scaffolds
Gene Expression Profiling
Regeneration (biology)
Biomaterial
Mesenchymal Stem Cells
X-Ray Microtomography
Alkaline Phosphatase
021001 nanoscience & nanotechnology
Rats
Microscopy, Electron, Scanning
Calcium
0210 nano-technology
Biomedical engineering
Subjects
Details
- ISSN :
- 15524981 and 15524973
- Volume :
- 109
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Materials Research Part B: Applied Biomaterials
- Accession number :
- edsair.doi.dedup.....eabb1a81e45b38d2637f2c3dfb6e9bc5