Back to Search
Start Over
Cotton GhSSI2 isoforms from the stearoyl acyl carrier protein fatty acid desaturase family regulate Verticillium wilt resistance
- Source :
- Molecular Plant Pathology
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Lipids are major and essential constituents of plant cells and provide energy for various metabolic processes. However, the function of the lipid signal in defence against Verticillium dahliae, a hemibiotrophic pathogen, remains unknown. Here, we characterized 19 conserved stearoyl‐ACP desaturase family proteins from upland cotton (Gossypium hirsutum). We further confirmed that GhSSI2 isoforms, including GhSSI2‐A, GhSSI2‐B, and GhSSI2‐C located on chromosomes A10, D10, and A12, respectively, played a dominant role to the cotton 18:1 (oleic acid) pool. Suppressing the expression of GhSSI2s reduced the 18:1 level, which autoactivated the hypersensitive response (HR) and enhanced cotton Verticillium wilt and Fusarium wilt resistance. We found that low 18:1 levels induced phenylalanine ammonia‐lyase‐mediated salicylic acid (SA) accumulation and activated a SA‐independent defence response in GhSSI2s‐silenced cotton, whereas suppressing expression of GhSSI2s affected PDF1.2‐dependent jasmonic acid (JA) perception but not the biosynthesis and signalling cascade of JA. Further investigation showed that structurally divergent resistance‐related genes and nitric oxide (NO) signal were activated in GhSSI2s‐silenced cotton. Taken together, these results indicate that SA‐independent defence response, multiple resistance‐related proteins, and elevated NO level play an important role in GhSSI2s‐regulated Verticillium wilt resistance. These findings broaden our knowledge regarding the lipid signal in disease resistance and provide novel insights into the molecular mechanism of cotton fungal disease resistance.<br />GhSACPD gene family isoforms GhSSI2‐A, GhSSI2‐B, and GhSSI2‐C displayed an important role in regulating 18:1 fatty acid level and negatively regulated cotton disease resistance to Verticillium wilt.
- Subjects :
- Fatty Acid Desaturases
0106 biological sciences
0301 basic medicine
Hypersensitive response
Verticillium wilt resistance
Gossypium hirsutum
Soil Science
Plant Science
GhSACPD
Verticillium
Plant disease resistance
01 natural sciences
Mixed Function Oxygenases
03 medical and health sciences
chemistry.chemical_compound
lipid signal
Gene Expression Regulation, Plant
Acyl Carrier Protein
Protein Isoforms
Verticillium dahliae
Molecular Biology
Disease Resistance
Plant Diseases
Plant Proteins
Gossypium
biology
Jasmonic acid
Fatty Acids
food and beverages
Original Articles
biology.organism_classification
Fusarium wilt
030104 developmental biology
Fatty acid desaturase
chemistry
Biochemistry
biology.protein
Original Article
molecular mechanism
Verticillium wilt
Agronomy and Crop Science
Salicylic acid
010606 plant biology & botany
Subjects
Details
- ISSN :
- 13643703 and 14646722
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Molecular Plant Pathology
- Accession number :
- edsair.doi.dedup.....eac8687ef6d4debcb2436170501c3a78